Electroporation Procedure for the Single-Copy Gene Expression in an Efflux-Deficient Bacterial Strain

Published: January 05, 2024

doi:

10.3791/201442

¹Department of Microbiology,University of Manitoba

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Electroporation PTNS2 Helper Plasmid PUC18-Tmini-Tn7-Tlac Gentamicin AdeIJK Insertion Plasmid Electrocompetent Cells LB Broth LB Agar Gentamicin Colony PCR ABglmS2 Forward Tn7 Reverse A.baumannii ATCC 17978 AB 258

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Electroporation Procedure for the Single-Copy Gene Expression in an Efflux-Deficient Bacterial Strain. J. Vis. Exp. (Pending Publication), e201442, doi:10.3791/201442 (2024).

Electroporation Procedure for the Single-Copy Gene Expression in an Efflux-Deficient Bacterial Strain

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