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JoVE Journal > Immunology and Infection
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Immunology and Infection
Electroporation Procedure for the Single-Copy Gene Expression in an Efflux-Deficient Bacterial Strain
Published: January 05, 2024
doi:
10.3791/201442
Dawn White
,
Ayush Kumar
1
Department of Microbiology
,
University of Manitoba
Tags
Electroporation
PTNS2 Helper Plasmid
PUC18-Tmini-Tn7-Tlac Gentamicin AdeIJK Insertion Plasmid
Electrocompetent Cells
LB Broth
LB Agar
Gentamicin
Colony PCR
ABglmS2 Forward
Tn7 Reverse
A.baumannii ATCC 17978
AB 258
check_url/201442?article_type=t
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Electroporation Procedure for the Single-Copy Gene Expression in an Efflux-Deficient Bacterial Strain.
J. Vis. Exp.
(Pending Publication), e201442, doi:10.3791/201442 (2024).
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