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JoVE Journal Bioengineering
Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface
 

Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface

Article DOI: 10.3791/3519-v • 13:22 min • November 2nd, 2011
November 2nd, 2011 •
1, 1
1Biomedical Engineering Department, Georgia Institute of Technology

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An adhesion frequency assay for measuring receptor-ligand interaction kinetics when both molecules are anchored on the surfaces of the interacting cells is described. This mechanically-based assay is exemplified using a micropipette-pressurized human red blood cell as adhesion sensor and integrin αLβ2 and intercellular adhesion molecule-1 as interacting receptors and ligands.

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Adhesion Frequency Assay In Situ Kinetics Analysis Cross-junctional Molecular Interactions Cell-cell Interface Micropipette Adhesion Assay Receptor-ligand Binding Kinetics Human Red Blood Cell (RBC) Micromanipulation Bond Formation Adhesion Event RBC Elongation Ligands Immobilized Adhesion Probability Binding Curve Probabilistic Model Receptor-ligand Reaction Kinetics 2D Affinity Off-rate Fcγ Receptors IgG Fc1-6 Selectins Glycoconjugate Ligands Integrins Homotypical Cadherin Binding T Cell Receptor Coreceptor Peptide-major Histocompatibility Complexes
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