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Journal / Immunology and Infection / Editing genetico delle cellule B del macaco…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Editing genetico delle cellule B del macaco rhesus primario
 
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Editing genetico delle cellule B del macaco rhesus primario

Article DOI: 10.3791/64858-v • 09:53 min • February 10th, 2023
February 10th, 2023 •
1, 2, 2, 3,5, 1, 1,6, 1, 2, 1,4
1Laboratory of Molecular Immunology, The Rockefeller University, 2Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 3Laboratory of Retrovirology, The Rockefeller University, 4Howard Hughes Medical Institute, The Rockefeller University, 5Laboratory of Applied Virology and Precision Medicine, King Abdullah University of Science and Technology (KAUST), 6Vaccine and Immunotherapy Center, Wistar Institute

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Presentiamo un metodo per la coltura e l'editing genetico delle cellule B primarie del macaco rhesus utilizzando CRISPR / Cas9 e il sierotipo 6 del virus adeno-associato ricombinante per lo studio delle terapie con cellule B.

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Questo mese in JoVE Numero 192 Cellule B editing genetico ingegneria del genoma CRISPR / Cas9 virus adeno-associati macaco rhesus primate non umano terapia cellulare
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