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Journal / Immunology and Infection / Genredigering av primære Rhesus Macaque B-celler
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Genredigering av primære Rhesus Macaque B-celler
 
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Genredigering av primære Rhesus Macaque B-celler

Article DOI: 10.3791/64858-v • 09:53 min • February 10th, 2023
February 10th, 2023 •
1, 2, 2, 3,5, 1, 1,6, 1, 2, 1,4
1Laboratory of Molecular Immunology, The Rockefeller University, 2Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 3Laboratory of Retrovirology, The Rockefeller University, 4Howard Hughes Medical Institute, The Rockefeller University, 5Laboratory of Applied Virology and Precision Medicine, King Abdullah University of Science and Technology (KAUST), 6Vaccine and Immunotherapy Center, Wistar Institute

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Vi presenterer en metode for dyrking og genredigering av primære rhesusmakake B-celler ved bruk av CRISPR/Cas9 og rekombinant adenoassosiert virus serotype 6 for studier av B-celleterapier.

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Denne måneden i JoVE utgave 192 B-celler genredigering genomteknikk CRISPR / Cas9 adenoassosiert virus rhesusmakak ikke-menneskelig primat celleterapi
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