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手动复用荧光免疫学的优化、设计和避免陷阱

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Krebsforschung

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JoVE Journal Krebsforschung

Optimization, Design and Avoiding Pitfalls in Manual Multiplex Fluorescent Immunohistochemistry

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手动复用荧光免疫学的优化、设计和避免陷阱

DOI:

10.3791/59915-v

•

09:15 min

•

July 26, 2019

•

Jenny Lazarus, Yagiz Akiska, Mirna Perusina Lanfranca, Lawrence Delrosario, Lei Sun, Daniel Long, Jiaqi Shi, Howard Crawford, Marina P. Di Magliano, Weiping Zou, Timothy Frankel

¹Department of Surgery,University of Michigan, ²Department of Molecular and Cellular Physiology,University of Michigan, ³Department of Pathology,University of Michigan

Kapitel

00:04Titel
00:55Deparaffinization and Rehydration
02:24Slide Staining
05:05DAPI Application
05:55Imaging of Monoplexes and Multiplex Preparation
07:18Results: Monoplex and Multiplex Optimization
08:39Conclusion

Summary

Automatische Übersetzung

多路荧光免疫组织化学是一项新兴技术,能够可视化完整形式固定的石蜡嵌入(FFPE)组织内的多种细胞类型。介绍了确保成功7色多路复用的指南,其中包含优化抗体和试剂、准备幻灯片、设计和避免常见问题的技巧的说明。

Tags

Keywords: Multiplex Fluorescent Immunohistochemistry Cell Identification Tumor Microenvironment Deparaffinizing Rehydrating Antigen Retrieval Primary Antibody Fluorophore DAPI

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