JoVE
JoVE
Ressourcen für Lehrende
Forschung
Verhalten
Biochemie
Biologie
Biotechnik
Krebsforschung
Chemie
Entwicklungsbiologie
Ingenieurwesen
Umwelt
Genetik
Immunologie und Infektion
Medizin
Neurowissenschaften
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Lehre
Biologie
Chemie
Clinical
Ingenieurwesen
Umweltwissenschaften
Pharmacology
Physik
Psychologie
Statistik
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Autoren
Bibliothekare
Gymnasien
Über uns
Sign-In
Anmelden
Kontakt
Forschung
JoVE Journal
JoVE Encyclopedia of Experiments
Lehre
JoVE Core
JoVE Science Education
JoVE Lab Manual
Gymnasien
DE
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
DE
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Forschung
Verhalten
Biochemie
Biotechnik
Biologie
Krebsforschung
Chemie
Entwicklungsbiologie
Ingenieurwesen
Umwelt
Genetik
Immunologie und Infektion
Medizin
Neurowissenschaften
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Lehre
Biologie
Chemie
Clinical
Ingenieurwesen
Umweltwissenschaften
Pharmacology
Physik
Psychologie
Statistik
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Ihrem Kurs zugeordnete Videos
Teacher Resources
Get in Touch
Instant Trial
Log In
DE
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biochemie
/
通过基因表达分析的改良方法从谷物种子获取高质量的转录数据
JoVE Journal
Biochemie
This content is Free Access.
JoVE Journal
Biochemie
Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
Please note that all translations are automatically generated.
Click here for the English version.
通过基因表达分析的改良方法从谷物种子获取高质量的转录数据
DOI:
10.3791/60602-v
•
07:18 min
•
May 21, 2020
•
Vito M. Butardo Jr.
,
Christiane Seiler
,
Nese Sreenivasulu
,
Markus Kuhlmann
1
Department of Chemistry and Biotechnology
,
Swinburne University of Technology
,
2
Department of Molecular Genetics, Heterosis Research Group
,
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
,
3
Grain Quality and Nutrition Center, Applied Functional Genomics Cluster
,
International Rice Research Institute
Kapitel
00:04
Introduction
00:40
Microarray Hybridization
03:09
Microarray Scanning
05:05
Results: Representative Transcriptome Data Analyses
06:39
Conclusion
Summary
Automatische Übersetzung
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatische Übersetzung
提出了谷物转录分析的方法。基于微阵列的基因表达分析从谷物中分离高质量的总RNA开始,并随着cDNA的生成而继续。在cRNA标签和微阵列杂交后,提出了信号检测和质量控制的建议。
Tags
Microarray Hybridization
Gene Expression Profiling
Cereal Seeds
Transcriptome Data
High-throughput Sequencing
Blocking Agent
Fragmentation Mix
Hybridization Buffer
Microarray Slide
Hybridization Assembly
Hybridization Oven
Article
Embed
ZUR WIEDERGABELISTE HINZUFÜGEN
Usage Statistics
Verwandte Videos
对于高度纯化RNA的种子定量转录组分析的隔离中使用的有效方法
从交联骨骼肌制备高质量核的基于过滤的方法进行染色质免疫沉淀
用等压标记、广泛的液相色谱法、质谱法和软件辅助定量技术进行深层蛋白质组分析
植物细胞悬浮液中咖啡因的提取、酶活性及咖啡因合成酶基因的表达
荧光显微镜竞争滴定法测定转录机-dna 结合感的高灵敏度
通过建立单罐双酶级联,从弗拉瓦内生物合成一种黄酮
血管紧张蛋白II图解的硝酸分析与鉴定
DNA序列识别利用高通量原酶分析的DNAPrimase
通过低温BN-MS分析进行高分辨率复杂分析
优质发酵鱼产品的制备
Read Article