Stuart Cordwell Mass Spectrometry Core Facility University of Sydney Biography Publications Institution JoVE Articles Stuart Cordwell has not added a biography. If you are Stuart Cordwell and would like to personalize this page please email our Author Liaison for assistance. Publications Diverse Peptide Hormones Affecting Root Growth Identified in the Medicago Truncatula Secreted Peptidome Molecular & Cellular Proteomics : MCP. Jan, 2018 | Pubmed ID: 29079721 Human Macrophage Cathepsin B-mediated C-terminal Cleavage of Apolipoprotein A-I at Ser228 Severely Impairs Antiatherogenic Capacity FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology. Dec, 2016 | Pubmed ID: 27630170 Cell Shaving and False-Positive Control Strategies Coupled to Novel Statistical Tools to Profile Gram-Positive Bacterial Surface Proteomes Methods in Molecular Biology (Clifton, N.J.). 2016 | Pubmed ID: 27311663 Ultrastructure of the Liver Microcirculation Influences Hepatic and Systemic Insulin Activity and Provides a Mechanism for Age-related Insulin Resistance Aging Cell. Aug, 2016 | Pubmed ID: 27095270 Proteomic Identification of Putative MicroRNA394 Target Genes in Arabidopsis Thaliana Identifies Major Latex Protein Family Members Critical for Normal Development Molecular & Cellular Proteomics : MCP. 06, 2016 | Pubmed ID: 27067051 Cellular Targets of the Myeloperoxidase-derived Oxidant Hypothiocyanous Acid (HOSCN) and Its Role in the Inhibition of Glycolysis in Macrophages Free Radical Biology & Medicine. 05, 2016 | Pubmed ID: 26898502 Comparative Analysis of Staphylococcus Epidermidis Strains Utilizing Quantitative and Cell Surface Shaving Proteomics Journal of Proteomics. Jan, 2016 | Pubmed ID: 26370163 Homogentisate 1-2-Dioxygenase Downregulation in the Chronic Persistence of Pseudomonas Aeruginosa Australian Epidemic Strain-1 in the CF Lung PloS One. 2015 | Pubmed ID: 26252386 Enrichment and Identification of Bacterial Glycopeptides by Mass Spectrometry Methods in Molecular Biology (Clifton, N.J.). 2015 | Pubmed ID: 25820733 Phosphorylation of Krüppel-like Factor 3 (KLF3/BKLF) and C-terminal Binding Protein 2 (CtBP2) by Homeodomain-interacting Protein Kinase 2 (HIPK2) Modulates KLF3 DNA Binding and Activity The Journal of Biological Chemistry. Mar, 2015 | Pubmed ID: 25659434 Global Analysis of Myocardial Peptides Containing Cysteines with Irreversible Sulfinic and Sulfonic Acid Post-translational Modifications Molecular & Cellular Proteomics : MCP. Mar, 2015 | Pubmed ID: 25561502 Comparative Proteomics and Glycoproteomics Reveal Increased N-linked Glycosylation and Relaxed Sequon Specificity in Campylobacter Jejuni NCTC11168 O Journal of Proteome Research. Nov, 2014 | Pubmed ID: 25093254 Structural Basis for Phosphorylation and Lysine Acetylation Cross-talk in a Kinase Motif Associated with Myocardial Ischemia and Cardioprotection The Journal of Biological Chemistry. Sep, 2014 | Pubmed ID: 25008320 Staphylococcus Aureus Surface Proteins Involved in Adaptation to Oxacillin Identified Using a Novel Cell Shaving Approach Journal of Proteome Research. Jun, 2014 | Pubmed ID: 24708102 Therapeutic Inflammatory Monocyte Modulation Using Immune-modifying Microparticles Science Translational Medicine. Jan, 2014 | Pubmed ID: 24431111 Beyond Gene Expression: the Impact of Protein Post-translational Modifications in Bacteria Journal of Proteomics. Jan, 2014 | Pubmed ID: 23994099 Site-specific Glycan-peptide Analysis for Determination of N-glycoproteome Heterogeneity Journal of Proteome Research. Dec, 2013 | Pubmed ID: 24090084 Modulation of Gene Expression by Pseudomonas Aeruginosa During Chronic Infection in the Adult Cystic Fibrosis Lung Microbiology (Reading, England). Nov, 2013 | Pubmed ID: 24014664 Secretome of Transmissible Pseudomonas Aeruginosa AES-1R Grown in a Cystic Fibrosis Lung-like Environment Journal of Proteome Research. Dec, 2013 | Pubmed ID: 23991618 Functional Decorations: Post-translational Modifications and Heart Disease Delineated by Targeted Proteomics Genome Medicine. 2013 | Pubmed ID: 23445784 Large-scale Capture of Peptides Containing Reversibly Oxidized Cysteines by Thiol-disulfide Exchange Applied to the Myocardial Redox Proteome Analytical Chemistry. Apr, 2013 | Pubmed ID: 23438843 Characterization of Reaction Conditions Providing Rapid and Specific Cysteine Alkylation for Peptide-based Mass Spectrometry Biochimica Et Biophysica Acta. Jan, 2013 | Pubmed ID: 22910378 Diversity in the Protein N-glycosylation Pathways Within the Campylobacter Genus Molecular & Cellular Proteomics : MCP. Nov, 2012 | Pubmed ID: 22859570 A Novel Method for the Simultaneous Enrichment, Identification, and Quantification of Phosphopeptides and Sialylated Glycopeptides Applied to a Temporal Profile of Mouse Brain Development Molecular & Cellular Proteomics : MCP. Nov, 2012 | Pubmed ID: 22843994 Modification of the Campylobacter Jejuni N-linked Glycan by EptC Protein-mediated Addition of Phosphoethanolamine The Journal of Biological Chemistry. Aug, 2012 | Pubmed ID: 22761430 Identification of a General O-linked Protein Glycosylation System in Acinetobacter Baumannii and Its Role in Virulence and Biofilm Formation PLoS Pathogens. 2012 | Pubmed ID: 22685409 Proteomic Profiling of Pseudomonas Aeruginosa AES-1R, PAO1 and PA14 Reveals Potential Virulence Determinants Associated with a Transmissible Cystic Fibrosis-associated Strain BMC Microbiology. 2012 | Pubmed ID: 22264352 Release of Tissue-specific Proteins into Coronary Perfusate As a Model for Biomarker Discovery in Myocardial Ischemia/Reperfusion Injury Journal of Proteome Research. Mar, 2012 | Pubmed ID: 22250753 Proteomics of Pseudomonas Aeruginosa Australian Epidemic Strain 1 (AES-1) Cultured Under Conditions Mimicking the Cystic Fibrosis Lung Reveals Increased Iron Acquisition Via the Siderophore Pyochelin Journal of Proteome Research. Feb, 2012 | Pubmed ID: 22054071 Phosphoproteomic Profiling of the Myocyte Circulation. Cardiovascular Genetics. Oct, 2011 | Pubmed ID: 22010164 Sequence TTKF ↓ QE Defines the Site of Proteolytic Cleavage in Mhp683 Protein, a Novel Glycosaminoglycan and Cilium Adhesin of Mycoplasma Hyopneumoniae The Journal of Biological Chemistry. Dec, 2011 | Pubmed ID: 21969369 Pseudomonas Aeruginosa AES-1 Exhibits Increased Virulence Gene Expression During Chronic Infection of Cystic Fibrosis Lung PloS One. 2011 | Pubmed ID: 21935417 Current Methodologies for Proteomics of Bacterial Surface-exposed and Cell Envelope Proteins Proteomics. Aug, 2011 | Pubmed ID: 21726045 Proteomics of the Oxidative Stress Response Induced by Hydrogen Peroxide and Paraquat Reveals a Novel AhpC-like Protein in Pseudomonas Aeruginosa Proteomics. Aug, 2011 | Pubmed ID: 21674802 Quantitative N-linked Glycoproteomics of Myocardial Ischemia and Reperfusion Injury Reveals Early Remodeling in the Extracellular Environment Molecular & Cellular Proteomics : MCP. Aug, 2011 | Pubmed ID: 21441315 Simultaneous Glycan-peptide Characterization Using Hydrophilic Interaction Chromatography and Parallel Fragmentation by CID, Higher Energy Collisional Dissociation, and Electron Transfer Dissociation MS Applied to the N-linked Glycoproteome of Campylobacter Jejuni Molecular & Cellular Proteomics : MCP. Feb, 2011 | Pubmed ID: 20360033 Proteomics of Bacterial Pathogens: Pseudomonas Aeruginosa Infections in Cystic Fibrosis - a Case Study Proteomics. Clinical Applications. Feb, 2010 | Pubmed ID: 21137046 Improved Accuracy of Cell Surface Shaving Proteomics in Staphylococcus Aureus Using a False-positive Control Proteomics. May, 2010 | Pubmed ID: 20217865 Mass Spectrometric Characterization of the Campylobacter Jejuni Adherence Factor CadF Reveals Post-translational Processing That Removes Immunogenicity While Retaining Fibronectin Binding Proteomics. Jan, 2010 | Pubmed ID: 19941310 Technologies for Plasma Membrane Proteomics Proteomics. Feb, 2010 | Pubmed ID: 19834916 Comparative Transcriptional and Translational Analysis of Leptospiral Outer Membrane Protein Expression in Response to Temperature PLoS Neglected Tropical Diseases. Dec, 2009 | Pubmed ID: 19997626 Mass Spectrometric Characterization of the Surface-associated 42 KDa Lipoprotein JlpA As a Glycosylated Antigen in Strains of Campylobacter Jejuni Journal of Proteome Research. Oct, 2009 | Pubmed ID: 19689120 Anti-tropomyosin Antibodies Co-localise with Actin Microfilaments and Label Plasmodesmata European Journal of Cell Biology. Jun, 2009 | Pubmed ID: 19328591 Campylobacter Proteomics: Guidelines, Challenges and Future Perspectives Expert Review of Proteomics. Feb, 2009 | Pubmed ID: 19210127 Mitochondria: A Mirror into Cellular Dysfunction in Heart Disease Proteomics. Clinical Applications. Jun, 2008 | Pubmed ID: 21136884 The Role of Proteomics in Clinical Cardiovascular Biomarker Discovery Molecular & Cellular Proteomics : MCP. Oct, 2008 | Pubmed ID: 18667414 Statistical Analysis of Image Data Provided by Two-dimensional Gel Electrophoresis for Discovery Proteomics Methods in Molecular Medicine. 2008 | Pubmed ID: 18453095 Sequential Extraction of Proteins by Chemical Reagents Methods in Molecular Biology (Clifton, N.J.). 2008 | Pubmed ID: 18369859 Identification of Membrane-associated Proteins from Campylobacter Jejuni Strains Using Complementary Proteomics Technologies Proteomics. Jan, 2008 | Pubmed ID: 18095373 Altered Proteins of the Anterior Cingulate Cortex White Matter Proteome in Schizophrenia Proteomics. Clinical Applications. Feb, 2007 | Pubmed ID: 21136665 Abnormal Pathways in the Genu of the Corpus Callosum in Schizophrenia Pathogenesis: a Proteome Study Proteomics. Clinical Applications. Oct, 2007 | Pubmed ID: 21136626 A Proteome Analysis of the Dorsolateral Prefrontal Cortex in Human Alcoholic Patients Proteomics. Clinical Applications. Jan, 2007 | Pubmed ID: 21136612 The Nuclear Proteome and DNA-binding Fraction of Human Raji Lymphoma Cells Biochimica Et Biophysica Acta. Apr, 2007 | Pubmed ID: 17369005 Proteomics of Ischemia and Reperfusion Injuries in Rabbit Myocardium with and Without Intervention by an Oxygen-free Radical Scavenger Proteomics. Dec, 2006 | Pubmed ID: 17133370 Effects of Chronic Risperidone Treatment on the Striatal Protein Profiles in Rats Brain Research. Oct, 2006 | Pubmed ID: 16938284 Technologies for Bacterial Surface Proteomics Current Opinion in Microbiology. Jun, 2006 | Pubmed ID: 16679049 Ischemia-specific Phosphorylation and Myofilament Translocation of Heat Shock Protein 27 Precedes Alpha B-crystallin and Occurs Independently of Reactive Oxygen Species in Rabbit Myocardium Journal of Molecular and Cellular Cardiology. Jun, 2006 | Pubmed ID: 16678850 Proteomic Identification of Putative Plasmodesmatal Proteins from Chara Corallina Proteomics. Jul, 2005 | Pubmed ID: 16075417 Proteomics of Ischemia/reperfusion Injury in Rabbit Myocardium Reveals Alterations to Proteins of Essential Functional Systems Proteomics. Apr, 2005 | Pubmed ID: 15800873 Modifications of Myosin-regulatory Light Chain Correlate with Function of Stunned Myocardium Journal of Molecular and Cellular Cardiology. Jul, 2003 | Pubmed ID: 12818574 Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows Matthew B. O'Rourke1,2, Matthew P. Padula2, Caine Smith3, Priscilla Youssef4, Stuart Cordwell1, Paul Witting4, Greg Sutherland3, Ben Crossett1 1Mass Spectrometry Core Facility, University of Sydney, 2Proteomics Core Facility, University of Technology Sydney, 3Neuropathology Group, Discipline of Pathology, School of Medical Sciences, University of Sydney, 4Redox Biology Group, Discipline of Pathology, School of Medical Sciences, University of Sydney JoVE 56778 Bioquímica
Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows Matthew B. O'Rourke1,2, Matthew P. Padula2, Caine Smith3, Priscilla Youssef4, Stuart Cordwell1, Paul Witting4, Greg Sutherland3, Ben Crossett1 1Mass Spectrometry Core Facility, University of Sydney, 2Proteomics Core Facility, University of Technology Sydney, 3Neuropathology Group, Discipline of Pathology, School of Medical Sciences, University of Sydney, 4Redox Biology Group, Discipline of Pathology, School of Medical Sciences, University of Sydney JoVE 56778 Bioquímica