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Biochemistry

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JoVE Journal Biochemistry
Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows
 

Optimal Preparation of Formalin Fixed Samples for Peptide Based Matrix Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Workflows

Article DOI: 10.3791/56778-v • 08:16 min • January 16th, 2018
January 16th, 2018 •
1,2, 2, 3, 4, 1, 4, 3, 1
1Mass Spectrometry Core Facility, University of Sydney, 2Proteomics Core Facility, University of Technology Sydney, 3Neuropathology Group, Discipline of Pathology, School of Medical Sciences, University of Sydney, 4Redox Biology Group, Discipline of Pathology, School of Medical Sciences, University of Sydney

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This protocol describes a reproducible and reliable method for the sublimation-based preparation of formalin fixed tissue destined for imaging mass spectrometry.

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Formalin Fixed Samples Peptide-based Matrix Assisted Laser Desorption/ionization Mass Spectrometry Imaging Workflows Tissue Sample Preparation Peptide Mass Spectrometry Imaging Nitrocellulose Coated Slides Vapor Chambers Custom Vapor Chambers Thick Blotting Paper Plastic Petri Dish FFPE Tissue Float Mount Xylene
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