一个生产缺血性损伤模型的简化技术
Summary
我们已经开发出一种微创技术，创建除以中央动脉和神经，颅神经血管束兔子缺血耳朵伤口模型。皮下隧道，然后切开皮下组织。此过程会导致皮肤的破坏最小，可以安全地用于糖尿病动物。
Abstract
目前糖尿病伤口研究中的一个主要障碍是缺乏缺血性损伤模型，可以安全地用于糖尿病动物。工作在非缺血性伤口的药物可能无法正常工作，在人类糖尿病患者的伤口，因为血管病变是一个主要因素，阻碍了这些伤口的愈合。我们在2007年发表的文章描述了一种微创外科技术创造一只兔子耳缺血性损伤模型。从那时起，我们已进一步简化，易于操作的过程。一只耳朵上，提出了三个小的皮肤切口上的血管蒂，从耳根1-2厘米。中央动脉结扎和切断神经。整个颅捆绑切断结扎，留下完整的只有尾分行。圆周皮下隧道，通过切口，以减少皮下组织，肌肉，神经，和小型船只。另一只耳朵被用来作为非缺血性的控制。腹侧方Ø四个伤口F各耳。这种技术产生配对比较4缺血性伤口和4个非缺血性伤口在一个动物。手术后，缺血耳朵是凉爽和紫绀，呈减少运动和耳动脉中的脉冲缺乏。缺血耳的皮肤温度为1-10°C下比正常耳，这种差异维持一个月以上。在缺血性比对照耳耳耳组织高能磷酸含量较低。伤口愈合时间长于在缺血性耳在非缺血性耳时使用了相同的待遇。该技术目前已超过80只，其中23例糖尿病（糖尿病的时间从2个星期至2年不等）。没有一个单一的兔子已开发的任何手术并发症，如出血，感染，或在皮肤切口破裂。该模型具有许多优点，如小皮中断，缺血时间较长，成功率较高，许多其他车型相比。它长约n是安全地使用在动物抵抗力降低，也可以修改，以满足不同的测试要求。
Protocol
Discussion
糖尿病的影响，在美国，2,3 23.6万人，有15-20％的患者发展足部溃疡，在其一生中，4,5在每年的治疗费用超过25亿美元。3,6然而，即使是最好的治疗愈合率只有50％，并实现这往往是暂时的，复发率高达66％的糖尿病患者7。3,6当溃疡发生在糖尿病患者的主要并发症，如感染和截肢的大幅度增加。尽管近一个世纪广泛的研究和发展成千上万的敷料产品，尚没有证明是有…
Divulgaciones
The authors have nothing to disclose.
Acknowledgements
部分支持这项研究是由NIH资助1RO1DK74566和1RO1AM52984。笔者想感谢她的帮助杰奎琳·麦卡锡女士在手术过程中，在RRC的DRS。剑谱王和Harshini的萨罗吉尼为帮助他们在一些兔子，荣丸为她组织学工作，博士和她的高效液相色谱法测量高能磷酸盐的李明女士的手术和术后护理。
Materials
For monitoring during surgery
- A heating pad to maintain normal temperature.
- A transcutaneous oxygen monitor.
- A temperature monitor.
For surgery
- A #3 knife with #15 blade.
- Two pairs of forceps (one for skin and another for other soft tissues).
- 2-3 pairs of small clamps.
- One or two pairs of micro scissors (for tissue cutting).
- One pair of small scissors (for cutting sutures).
- A needle holder.
- A few stainless steel punches (for making wounds).
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