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快速PCR热循环利用微型热对流
JoVE Revista
Biología
This content is Free Access.
JoVE Revista Biología
Rapid PCR Thermocycling using Microscale Thermal Convection
DOI:

09:02 min

March 05, 2011

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Capítulos

  • 00:05Título
  • 01:21Basic Design of the Convective Thermocycler
  • 02:48Preparation and Loading of the PCR Reaction Mixture
  • 03:42Running the Reaction in the Convective Thermocycler
  • 04:25Performing Gel Electrophoresis Analysis
  • 05:31Representative Results for Rapid PCR Thermocycling Using Microscale Thermal Convection
  • 08:33Conclusion

Summary

Traducción Automática

我们描述了一种新的方法,通过聚合酶链反应(PCR)进行DNA复制。热对流是利用变性,退火,以及延长条件之间不断穿梭试剂保持反对在反应器的表面温度恒定。这在本质上简单设计的承诺,使快速PCR更方便。

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