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JoVE Journal Neuroscience
Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons
 

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

Article DOI: 10.3791/61434-v • 14:02 min • October 31st, 2020
October 31st, 2020 •
1, 1, 1, 2, 2, 2, 1, 2,3, 1
1Department of Molecular Biochemistry and Pharmacology, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 2Department of Neuroscience, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, 3Dulbecco Telethon Institute, Istituto di Ricerche Farmacologiche Mario Negri IRCCS

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This protocol shows how to employ super-resolution microscopy to study protein co-localization in primary neuronal cultures.

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Super-resolution Imaging Co-localization Proteins Synaptic Markers Primary Neurons Neurodegenerative Disorders Neurological Disorders Imaging Studies Function And Plasticity High-resolution Imaging Techniques Structured Illumination Microscopy SIM Widefield Microscopy Nanometers Controls Statistical Methods Analysis Of Imaging Data Reagents Chambered Coverslips Mounting Solutions Objective Mouse Hippocampal Primary Neuron
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