Arek Kulczyk Institute for Quantitative Biomedicine & Department of Biochemistry Rutgers University Biography Publications Institution JoVE Articles Arek Kulczyk Arek Kulczyk is an Assistant Professor in the Institute for Quantitative Biomedicine and the Department of Biochemistry at Rutgers University. The primary research goal of the Kulczyk Laboratory is to integrate structural approaches, in particular single-particle cryo-electron microscopy (cryo-EM), single-molecule techniques and novel correlative light and electron microscopy (CLEM) methods to study DNA replication and repair in human mitochondria.Arek earned his Ph.D. from the University of Cambridge in the UK. At the MRC Laboratory of Molecular Biology, Arek worked on NMR structure determination of zinc-finger domains from DNA ligase III (DL3) and poly-ADP ribose polymerase 1 (PARP-1), the two medically important proteins involved in base excision repair of human DNA. After earning his doctorate degree, he moved to Boston, MA and began postdoctoral studies in the field of DNA replication at Harvard University. Under mentorship of Charles Richardson, he developed novel single-molecule techniques for monitoring enzymatic activities of the replication proteins. After completing his postdoctoral training, Arek became an Instructor in Biological Chemistry and Molecular Pharmacology at Harvard Medical School in Boston. This junior faculty appointment provided him an opportunity to establish an independent research program. Arek determined a structure of the 650 kDa bacteriophage T7 replisome using single-particle cryo-EM. The structure provided the first detailed snapshot of the entire replisome and revealed fundamental molecular mechanisms for coordination of leading- and lagging-strand synthesis. Publications Coupling DTTP Hydrolysis with DNA Unwinding by the DNA Helicase of Bacteriophage T7 The Journal of Biological Chemistry. Sep, 2011 | Pubmed ID: 21840995 Pyrovanadolysis, a Pyrophosphorolysis-like Reaction Mediated by Pyrovanadate, Mn2+, and DNA Polymerase of Bacteriophage T7 The Journal of Biological Chemistry. Aug, 2011 | Pubmed ID: 21697085 Simultaneous Single-molecule Measurements of Phage T7 Replisome Composition and Function Reveal the Mechanism of Polymerase Exchange Proceedings of the National Academy of Sciences of the United States of America. Mar, 2011 | Pubmed ID: 21245349 Conformational Dynamics of Bacteriophage T7 DNA Polymerase and Its Processivity Factor, Escherichia Coli Thioredoxin Proceedings of the National Academy of Sciences of the United States of America. Aug, 2010 | Pubmed ID: 20696935 Inadequate Inhibition of Host RNA Polymerase Restricts T7 Bacteriophage Growth on Hosts Overexpressing Udk Molecular Microbiology. Jan, 2008 | Pubmed ID: 18067538 Solution Structure and DNA Binding of the Zinc-finger Domain from DNA Ligase IIIalpha Journal of Molecular Biology. Aug, 2004 | Pubmed ID: 15288782 Um fluxo de trabalho robusto de crio-elétron de partículas únicas (crio-EM) com cryoSPARC, RELION e Scipion Megan C. DiIorio1, Arkadiusz W. Kulczyk1,2 1Institute for Quantitative Biomedicine, Rutgers University, 2Department of Biochemistry & Microbiology, Rutgers University JoVE 63387 Biochimie A observação direta de Enzimas DNA Replicação Usando um DNA molécula única Alongamento Assay Arkadiusz W. Kulczyk1, Nathan A. Tanner1, Joseph J. Loparo1, Charles C. Richardson1, Antoine M. van Oijen1 1Harvard Medical School JoVE 1689 Biologie
Um fluxo de trabalho robusto de crio-elétron de partículas únicas (crio-EM) com cryoSPARC, RELION e Scipion Megan C. DiIorio1, Arkadiusz W. Kulczyk1,2 1Institute for Quantitative Biomedicine, Rutgers University, 2Department of Biochemistry & Microbiology, Rutgers University JoVE 63387 Biochimie
A observação direta de Enzimas DNA Replicação Usando um DNA molécula única Alongamento Assay Arkadiusz W. Kulczyk1, Nathan A. Tanner1, Joseph J. Loparo1, Charles C. Richardson1, Antoine M. van Oijen1 1Harvard Medical School JoVE 1689 Biologie