Diogo E.S. Nogueira iBB - Institute for Bioengineering and Biosciences Instituto Superior Técnico, University of Lisbon Biography Publications Institution JoVE Articles Diogo E.S. NogueiraPhD student One of the main bottlenecks in the translation of stem cells to therapy is the difficulty in generating clinical numbers of these cells. 2D protocols, which are still widely used in culture of human induced pluripotent stem cells (hiPSCs) and their derivatives, are very labour intensive and expose cells to different conditions than those they will be subjected in vivo. As such, I am particularly interested in how to adapt standard Bioprocess Engineering principles to stem cells, in order to be able to produce clinical-grade and scale derivatives of hiPSCs with the potential to be used a wide range of biomedical applications. In particular, my work consists in the characterisation of novel bioreactor configurations and in their usage for highly efficient differentiation of hiPSCs, integrated, if necessary, with downstream processing of these cells for removal of undifferentiated hiPSCs with tumorigenic potential, along with other contaminant cell types, whose effects in the target organs are unpredictable. Publications Strategies for the Expansion of Human Induced Pluripotent Stem Cells As Aggregates in Single-use Vertical-Wheel™ Bioreactors Journal of Biological Engineering. Month, 2019 | Pubmed ID: 31534477 İnsan Pluripotent Kök Hücrelerinden Olgun Serebellar Organoidlerin Ölçeklenebilir Üretimi ve İmmünboyama ile Karakterizasyonu Teresa P. Silva1,2, Tiago G. Fernandes1, Diogo E. S. Nogueira1, Carlos A. V. Rodrigues1, Evguenia P. Bekman1,2,3, Yas Hashimura4, Sunghoon Jung4, Brian Lee4, Maria Carmo-Fonseca2, Joaquim M. S. Cabral1 1iBB - Institute for Bioengineering and Biosciences and Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, 2Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 3The Discoveries Centre for Regenerative and Precision Medicine, Lisbon Campus, Universidade de Lisboa, 4PBS Biotech, Inc, Camarillo, CA, USA JoVE 61143 Bio-ingénierie
İnsan Pluripotent Kök Hücrelerinden Olgun Serebellar Organoidlerin Ölçeklenebilir Üretimi ve İmmünboyama ile Karakterizasyonu Teresa P. Silva1,2, Tiago G. Fernandes1, Diogo E. S. Nogueira1, Carlos A. V. Rodrigues1, Evguenia P. Bekman1,2,3, Yas Hashimura4, Sunghoon Jung4, Brian Lee4, Maria Carmo-Fonseca2, Joaquim M. S. Cabral1 1iBB - Institute for Bioengineering and Biosciences and Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, 2Instituto de Medicina Molecular João Lobo Antunes, Faculdade de Medicina, Universidade de Lisboa, 3The Discoveries Centre for Regenerative and Precision Medicine, Lisbon Campus, Universidade de Lisboa, 4PBS Biotech, Inc, Camarillo, CA, USA JoVE 61143 Bio-ingénierie