Eric Gouaux Vollum Institute Oregon Health & Science University Biography Publications Institution JoVE Articles Eric Gouaux has not added a biography. If you are Eric Gouaux and would like to personalize this page please email our Author Liaison for assistance. Publications Structure and Symmetry Inform Gating Principles of Ionotropic Glutamate Receptors Neuropharmacology. Jan, 2017 | Pubmed ID: 27663701 X-ray Structures Define Human P2X3 Receptor Gating Cycle and Antagonist Action Nature. Sep, 2016 | Pubmed ID: 27626375 Architecture of Fully Occupied GluA2 AMPA Receptor-TARP Complex Elucidated by Cryo-EM Nature. Aug, 2016 | Pubmed ID: 27368053 Mechanism of NMDA Receptor Inhibition and Activation Cell. Apr, 2016 | Pubmed ID: 27062927 X-ray Structures and Mechanism of the Human Serotonin Transporter Nature. Apr, 2016 | Pubmed ID: 27049939 Thermostabilization of the Human Serotonin Transporter in an Antidepressant-Bound Conformation PloS One. 2015 | Pubmed ID: 26695939 Glycine Receptor Mechanism Elucidated by Electron Cryo-microscopy Nature. Oct, 2015 | Pubmed ID: 26344198 Neurotransmitter and Psychostimulant Recognition by the Dopamine Transporter Nature. May, 2015 | Pubmed ID: 25970245 X-ray Structures of Drosophila Dopamine Transporter in Complex with Nisoxetine and Reboxetine Nature Structural & Molecular Biology. Jun, 2015 | Pubmed ID: 25961798 Screening and Large-scale Expression of Membrane Proteins in Mammalian Cells for Structural Studies Nature Protocols. Nov, 2014 | Pubmed ID: 25299155 X-ray Structures of GluCl in Apo States Reveal a Gating Mechanism of Cys-loop Receptors Nature. Aug, 2014 | Pubmed ID: 25143115 Structure and Dynamics of AMPA Receptor GluA2 in Resting, Pre-open, and Desensitized States Cell. Aug, 2014 | Pubmed ID: 25109876 X-ray Structures of AMPA Receptor-cone Snail Toxin Complexes Illuminate Activation Mechanism Science (New York, N.Y.). Aug, 2014 | Pubmed ID: 25103405 NMDA Receptor Structures Reveal Subunit Arrangement and Pore Architecture Nature. Jul, 2014 | Pubmed ID: 25008524 X-ray Structure of Acid-sensing Ion Channel 1-snake Toxin Complex Reveals Open State of a Na(+)-selective Channel Cell. Feb, 2014 | Pubmed ID: 24507937 How LeuT Shapes Our Understanding of the Mechanisms of Sodium-coupled Neurotransmitter Transporters The Journal of Physiology. Mar, 2014 | Pubmed ID: 23878376 Structural Basis for Action by Diverse Antidepressants on Biogenic Amine Transporters Nature. Nov, 2013 | Pubmed ID: 24121440 X-ray Structure of Dopamine Transporter Elucidates Antidepressant Mechanism Nature. Nov, 2013 | Pubmed ID: 24037379 Novel Dicarboxylate Selectivity in an Insect Glutamate Transporter Homolog PloS One. 2013 | Pubmed ID: 23951049 Unanticipated Parallels in Architecture and Mechanism Between ATP-gated P2X Receptors and Acid Sensing Ion Channels Current Opinion in Structural Biology. Apr, 2013 | Pubmed ID: 23628284 A Fluorescence-detection Size-exclusion Chromatography-based Thermostability Assay for Membrane Protein Precrystallization Screening Structure (London, England : 1993). Aug, 2012 | Pubmed ID: 22884106 Structural Plasticity and Dynamic Selectivity of Acid-sensing Ion Channel-spider Toxin Complexes Nature. Sep, 2012 | Pubmed ID: 22842900 Substrate Binds in the S1 Site of the F253A Mutant of LeuT, a Neurotransmitter Sodium Symporter Homologue EMBO Reports. Sep, 2012 | Pubmed ID: 22836580 Molecular Mechanism of ATP Binding and Ion Channel Activation in P2X Receptors Nature. May, 2012 | Pubmed ID: 22535247 Structures of LeuT in Bicelles Define Conformation and Substrate Binding in a Membrane-like Context Nature Structural & Molecular Biology. Jan, 2012 | Pubmed ID: 22245965 X-ray Structures of LeuT in Substrate-free Outward-open and Apo Inward-open States Nature. Jan, 2012 | Pubmed ID: 22230955 Insights into Transport Mechanism from LeuT Engineered to Transport Tryptophan The EMBO Journal. Jan, 2012 | Pubmed ID: 21952050 Principles of Activation and Permeation in an Anion-selective Cys-loop Receptor Nature. Jun, 2011 | Pubmed ID: 21572436 Amino Terminal Domains of the NMDA Receptor Are Organized As Local Heterodimers PloS One. Apr, 2011 | Pubmed ID: 21544205 Neurotransmitter/sodium Symporter Orthologue LeuT Has a Single High-affinity Substrate Site Nature. Dec, 2010 | Pubmed ID: 21179170 Dynamic Superresolution Imaging of Endogenous Proteins on Living Cells at Ultra-high Density Biophysical Journal. Aug, 2010 | Pubmed ID: 20713016 The New York Consortium on Membrane Protein Structure (NYCOMPS): a High-throughput Platform for Structural Genomics of Integral Membrane Proteins Journal of Structural and Functional Genomics. Sep, 2010 | Pubmed ID: 20690043 Molecular Bases of Cyclodextrin Adapter Interactions with Engineered Protein Nanopores Proceedings of the National Academy of Sciences of the United States of America. May, 2010 | Pubmed ID: 20400691 X-ray Structure, Symmetry and Mechanism of an AMPA-subtype Glutamate Receptor Nature. Dec, 2009 | Pubmed ID: 19946266 Pore Architecture and Ion Sites in Acid-sensing Ion Channels and P2X Receptors Nature. Jul, 2009 | Pubmed ID: 19641589 Crystal Structure of the ATP-gated P2X(4) Ion Channel in the Closed State Nature. Jul, 2009 | Pubmed ID: 19641588 Structure and Mechanism of a Na+-independent Amino Acid Transporter Science (New York, N.Y.). Aug, 2009 | Pubmed ID: 19608859 Crystal Structure and Association Behaviour of the GluR2 Amino-terminal Domain The EMBO Journal. Jun, 2009 | Pubmed ID: 19461580 Unlocking the Molecular Secrets of Sodium-coupled Transporters Nature. May, 2009 | Pubmed ID: 19458710 Review. The Molecular Logic of Sodium-coupled Neurotransmitter Transporters Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences. Jan, 2009 | Pubmed ID: 18977735 A Competitive Inhibitor Traps LeuT in an Open-to-out Conformation Science (New York, N.Y.). Dec, 2008 | Pubmed ID: 19074341 Structure of Acid-sensing Ion Channel 1 at 1.9 A Resolution and Low PH Nature. Sep, 2007 | Pubmed ID: 17882215 Antidepressant Binding Site in a Bacterial Homologue of Neurotransmitter Transporters Nature. Aug, 2007 | Pubmed ID: 17687333 Coupling Substrate and Ion Binding to Extracellular Gate of a Sodium-dependent Aspartate Transporter Nature. Jan, 2007 | Pubmed ID: 17230192 Measurement of Conformational Changes Accompanying Desensitization in an Ionotropic Glutamate Receptor Cell. Oct, 2006 | Pubmed ID: 17018279 Fluorescence-detection Size-exclusion Chromatography for Precrystallization Screening of Integral Membrane Proteins Structure (London, England : 1993). Apr, 2006 | Pubmed ID: 16615909 Principles of Selective Ion Transport in Channels and Pumps Science (New York, N.Y.). Dec, 2005 | Pubmed ID: 16322449 Subunit Arrangement and Function in NMDA Receptors Nature. Nov, 2005 | Pubmed ID: 16281028 Mechanism of Positive Allosteric Modulators Acting on AMPA Receptors The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Sep, 2005 | Pubmed ID: 16192394 Membrane Proteins--pumping Along Current Opinion in Structural Biology. Aug, 2005 | Pubmed ID: 16043337 Crystal Structure of a Bacterial Homologue of Na+/Cl--dependent Neurotransmitter Transporters Nature. Sep, 2005 | Pubmed ID: 16041361 Mechanism of Partial Agonist Action at the NR1 Subunit of NMDA Receptors Neuron. Jul, 2005 | Pubmed ID: 15996549 Crystal Structure of the Vibrio Cholerae Cytolysin (VCC) Pro-toxin and Its Assembly into a Heptameric Transmembrane Pore Journal of Molecular Biology. Jul, 2005 | Pubmed ID: 15978620 AMPA Receptor Binding Cleft Mutations That Alter Affinity, Efficacy, and Recovery from Desensitization The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Apr, 2005 | Pubmed ID: 15829627 Structure of a Glutamate Transporter Homologue from Pyrococcus Horikoshii Nature. Oct, 2004 | Pubmed ID: 15483603 High Resolution Crystallographic Studies of Alpha-hemolysin-phospholipid Complexes Define Heptamer-lipid Head Group Interactions: Implication for Understanding Protein-lipid Interactions Protein Science : a Publication of the Protein Society. Jun, 2004 | Pubmed ID: 15152085 Structure and Function of AMPA Receptors The Journal of Physiology. Jan, 2004 | Pubmed ID: 14645452 Trimeric Subunit Stoichiometry of the Glutamate Transporters from Bacillus Caldotenax and Bacillus Stearothermophilus Biochemistry. Nov, 2003 | Pubmed ID: 14596613 Structural Basis for Modulation and Agonist Specificity of HCN Pacemaker Channels Nature. Sep, 2003 | Pubmed ID: 12968185 Structural Basis for Partial Agonist Action at Ionotropic Glutamate Receptors Nature Neuroscience. Aug, 2003 | Pubmed ID: 12872125 Mechanisms of Activation, Inhibition and Specificity: Crystal Structures of the NMDA Receptor NR1 Ligand-binding Core The EMBO Journal. Jun, 2003 | Pubmed ID: 12805203 Probing the Function, Conformational Plasticity, and Dimer-dimer Contacts of the GluR2 Ligand-binding Core: Studies of 5-substituted Willardiines and GluR2 S1S2 in the Crystal Biochemistry. May, 2003 | Pubmed ID: 12731861 Tuning Activation of the AMPA-sensitive GluR2 Ion Channel by Genetic Adjustment of Agonist-induced Conformational Changes Proceedings of the National Academy of Sciences of the United States of America. May, 2003 | Pubmed ID: 12730367 Arresting and Releasing Staphylococcal Alpha-hemolysin at Intermediate Stages of Pore Formation by Engineered Disulfide Bonds Protein Science : a Publication of the Protein Society. May, 2003 | Pubmed ID: 12717022 Three-dimensional Structure of the Ligand-binding Core of GluR2 in Complex with the Agonist (S)-ATPA: Implications for Receptor Subunit Selectivity Journal of Medicinal Chemistry. Feb, 2003 | Pubmed ID: 12593667 Vibrio Cholerae Cytolysin is Composed of an Alpha-hemolysin-like Core Protein Science : a Publication of the Protein Society. Feb, 2003 | Pubmed ID: 12538902 Competitive Antagonism of AMPA Receptors by Ligands of Different Classes: Crystal Structure of ATPO Bound to the GluR2 Ligand-binding Core, in Comparison with DNQX Journal of Medicinal Chemistry. Jan, 2003 | Pubmed ID: 12519060 Beta-barrel Membrane Protein Folding and Structure Viewed Through the Lens of Alpha-hemolysin Biochimica Et Biophysica Acta. Jan, 2003 | Pubmed ID: 12507754 Mechanism of Activation and Selectivity in a Ligand-gated Ion Channel: Structural and Functional Studies of GluR2 and Quisqualate Biochemistry. Dec, 2002 | Pubmed ID: 12501192 Structure-based Functional Design of Chemical Ligands for AMPA-subtype Glutamate Receptors Journal of Molecular Neuroscience : MN. Aug-Oct, 2002 | Pubmed ID: 12212767 Mechanism of Glutamate Receptor Desensitization Nature. May, 2002 | Pubmed ID: 12015593 A Vibrational Spectroscopic Investigation of Interactions of Agonists with GluR0, a Prokaryotic Glutamate Receptor Biochemistry. Feb, 2002 | Pubmed ID: 11814354 [4] Membrane Protein Crystallization: Application of Sparse Matrices to the α-hemolysin Heptamer Methods in Enzymology. 1997 | Pubmed ID: 27799118 Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram Jonathan A. Coleman1, Evan M. Green2, Eric Gouaux1,3 1Vollum Institute, Oregon Health & Science University, 2Graduate Group in Biophysics, University of California, San Francisco, 3Howard Hughes Medical Institute, Oregon Health & Science University JoVE 54792 Biochimie
Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram Jonathan A. Coleman1, Evan M. Green2, Eric Gouaux1,3 1Vollum Institute, Oregon Health & Science University, 2Graduate Group in Biophysics, University of California, San Francisco, 3Howard Hughes Medical Institute, Oregon Health & Science University JoVE 54792 Biochimie