Kevin Eggan Stanley Center for Psychiatric Research Broad Institute of MIT and Harvard Biography Publications Institution JoVE Articles Kevin Eggan has not added a biography. If you are Kevin Eggan and would like to personalize this page please email our Author Liaison for assistance. Publications A Multiplexed GRNA PiggyBac Transposon System Facilitates Efficient Induction of CRISPRi and CRISPRa in Human Pluripotent Stem Cells Scientific Reports. Jan, 2020 | Pubmed ID: 31959800 Publisher Correction: Exome Sequencing in Amyotrophic Lateral Sclerosis Implicates a Novel Gene, DNAJC7, Encoding a Heat-shock Protein Nature Neuroscience. Feb, 2020 | Pubmed ID: 31857710 Herpesviral Lytic Gene Functions Render the Viral Genome Susceptible to Novel Editing by CRISPR/Cas9 ELife. 12, 2019 | Pubmed ID: 31789594 Exome Sequencing in Amyotrophic Lateral Sclerosis Implicates a Novel Gene, DNAJC7, Encoding a Heat-shock Protein Nature Neuroscience. 12, 2019 | Pubmed ID: 31768050 RNA-seq As a Tool for Evaluating Human Embryo Competence Genome Research. 10, 2019 | Pubmed ID: 31548358 Cancer-Related Mutations Identified in Primed and Naive Human Pluripotent Stem Cells Cell Stem Cell. Oct, 2019 | Pubmed ID: 31543368 A High-Content Screen Identifies TPP1 and Aurora B As Regulators of Axonal Mitochondrial Transport Cell Reports. Sep, 2019 | Pubmed ID: 31533043 Comparison of Three Congruent Patient-specific Cell Types for the Modelling of a Human Genetic Schwann-cell Disorder Nature Biomedical Engineering. 07, 2019 | Pubmed ID: 30962586 Dysregulated Protocadherin-pathway Activity As an Intrinsic Defect in Induced Pluripotent Stem Cell-derived Cortical Interneurons from Subjects with Schizophrenia Nature Neuroscience. 02, 2019 | Pubmed ID: 30664768 ALS-implicated Protein TDP-43 Sustains Levels of STMN2, a Mediator of Motor Neuron Growth and Repair Nature Neuroscience. 02, 2019 | Pubmed ID: 30643292 A Stem Cell-Based Screening Platform Identifies Compounds That Desensitize Motor Neurons to Endoplasmic Reticulum Stress Molecular Therapy : the Journal of the American Society of Gene Therapy. 01, 2019 | Pubmed ID: 30446391 Convergence of Independent DISC1 Mutations on Impaired Neurite Growth Via Decreased UNC5D Expression Translational Psychiatry. 11, 2018 | Pubmed ID: 30410030 Oligodendrocyte Differentiation of Induced Pluripotent Stem Cells Derived from Subjects with Schizophrenias Implicate Abnormalities in Development Translational Psychiatry. 10, 2018 | Pubmed ID: 30352993 Comparative Genomic Analysis of Embryonic, Lineage-converted and Stem Cell-derived Motor Neurons Development (Cambridge, England). 11, 2018 | Pubmed ID: 30337375 All-optical Synaptic Electrophysiology Probes Mechanism of Ketamine-induced Disinhibition Nature Methods. 10, 2018 | Pubmed ID: 30275587 Hippo Signaling in the Ovary and Polycystic Ovarian Syndrome Journal of Assisted Reproduction and Genetics. Oct, 2018 | Pubmed ID: 30120633 Dipeptide Repeat Proteins Activate a Heat Shock Response Found in C9ORF72-ALS/FTLD Patients Acta Neuropathologica Communications. 07, 2018 | Pubmed ID: 29973287 The C9orf72-interacting Protein Smcr8 is a Negative Regulator of Autoimmunity and Lysosomal Exocytosis Genes & Development. 07, 2018 | Pubmed ID: 29950492 TDP-43 Induces P53-mediated Cell Death of Cortical Progenitors and Immature Neurons Scientific Reports. 05, 2018 | Pubmed ID: 29802307 Combining NGN2 Programming with Developmental Patterning Generates Human Excitatory Neurons with NMDAR-Mediated Synaptic Transmission Cell Reports. 05, 2018 | Pubmed ID: 29791859 All-Optical Electrophysiology for High-Throughput Functional Characterization of a Human IPSC-Derived Motor Neuron Model of ALS Stem Cell Reports. 06, 2018 | Pubmed ID: 29779896 Publisher Correction: Whole Genome Sequencing in Psychiatric Disorders: the WGSPD Consortium Nature Neuroscience. Jul, 2018 | Pubmed ID: 29549319 Whole Genome Sequencing in Psychiatric Disorders: the WGSPD Consortium Nature Neuroscience. 12, 2017 | Pubmed ID: 29184211 Generation of a Motor Nerve Organoid with Human Stem Cell-Derived Neurons Stem Cell Reports. 11, 2017 | Pubmed ID: 29107592 A Scaled Framework for CRISPR Editing of Human Pluripotent Stem Cells to Study Psychiatric Disease Stem Cell Reports. 10, 2017 | Pubmed ID: 29020615 Reactive Astrocytes Promote ALS-like Degeneration and Intracellular Protein Aggregation in Human Motor Neurons by Disrupting Autophagy Through TGF-β1 Stem Cell Reports. Aug, 2017 | Pubmed ID: 28712846 Assessing the Safety of Human Pluripotent Stem Cells and Their Derivatives for Clinical Applications Stem Cell Reports. Jul, 2017 | Pubmed ID: 28700896 Human Pluripotent Stem Cells Recurrently Acquire and Expand Dominant Negative P53 Mutations Nature. 05, 2017 | Pubmed ID: 28445466 Genetic Ablation of AXL Does Not Protect Human Neural Progenitor Cells and Cerebral Organoids from Zika Virus Infection Cell Stem Cell. Dec, 2016 | Pubmed ID: 27912091 Generation of a TLE3 Heterozygous Knockout Human Embryonic Stem Cell Line Using CRISPR-Cas9 Stem Cell Research. Sep, 2016 | Pubmed ID: 27879221 Generation of a TLE1 Homozygous Knockout Human Embryonic Stem Cell Line Using CRISPR-Cas9 Stem Cell Research. Sep, 2016 | Pubmed ID: 27879218 Two Familial ALS Proteins Function in Prevention/repair of Transcription-associated DNA Damage Proceedings of the National Academy of Sciences of the United States of America. Nov, 2016 | Pubmed ID: 27849576 Monitoring Peripheral Nerve Degeneration in ALS by Label-free Stimulated Raman Scattering Imaging Nature Communications. Oct, 2016 | Pubmed ID: 27796305 Comprehensive Protocols for CRISPR/Cas9-based Gene Editing in Human Pluripotent Stem Cells Current Protocols in Stem Cell Biology. Aug, 2016 | Pubmed ID: 27532820 Loss-of-function Mutations in the C9ORF72 Mouse Ortholog Cause Fatal Autoimmune Disease Science Translational Medicine. Jul, 2016 | Pubmed ID: 27412785 CAT7 and Cat7l Long Non-coding RNAs Tune Polycomb Repressive Complex 1 Function During Human and Zebrafish Development The Journal of Biological Chemistry. Sep, 2016 | Pubmed ID: 27405765 Modeling ALS with Motor Neurons Derived from Human Induced Pluripotent Stem Cells Nature Neuroscience. Apr, 2016 | Pubmed ID: 27021939 SLC52A3, A Brown-Vialetto-van Laere Syndrome Candidate Gene is Essential for Mouse Development, but Dispensable for Motor Neuron Differentiation Human Molecular Genetics. May, 2016 | Pubmed ID: 26976849 A Perspective on Stem Cell Modeling of Amyotrophic Lateral Sclerosis Cell Cycle (Georgetown, Tex.). 2015 | Pubmed ID: 26505672 Automated, High-throughput Derivation, Characterization and Differentiation of Induced Pluripotent Stem Cells Nature Methods. Sep, 2015 | Pubmed ID: 26237226 From Dish to Bedside: Lessons Learned While Translating Findings from a Stem Cell Model of Disease to a Clinical Trial Cell Stem Cell. Jul, 2015 | Pubmed ID: 26140603 Efficient CRISPR-Cas9-mediated Generation of Knockin Human Pluripotent Stem Cells Lacking Undesired Mutations at the Targeted Locus Cell Reports. May, 2015 | Pubmed ID: 25937281 Focus on Induced Pluripotency and Cellular Reprogramming The EMBO Journal. Jun, 2015 | Pubmed ID: 25916828 Generation of Neuropeptidergic Hypothalamic Neurons from Human Pluripotent Stem Cells Development (Cambridge, England). Feb, 2015 | Pubmed ID: 25670790 ALS-causative Mutations in FUS/TLS Confer Gain and Loss of Function by Altered Association with SMN and U1-snRNP Nature Communications. 2015 | Pubmed ID: 25625564 Motoneurons Derived from Induced Pluripotent Stem Cells Develop Mature Phenotypes Typical of Endogenous Spinal Motoneurons The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Jan, 2015 | Pubmed ID: 25609642 Modeling Pain in Vitro Using Nociceptor Neurons Reprogrammed from Fibroblasts Nature Neuroscience. Jan, 2015 | Pubmed ID: 25420066 IPSC-derived Dopamine Neurons Reveal Differences Between Monozygotic Twins Discordant for Parkinson's Disease Cell Reports. Nov, 2014 | Pubmed ID: 25456120 Genetic Variation in Human DNA Replication Timing Cell. Nov, 2014 | Pubmed ID: 25416942 The Role of Maternal-specific H3K9me3 Modification in Establishing Imprinted X-chromosome Inactivation and Embryogenesis in Mice Nature Communications. 2014 | Pubmed ID: 25394724 Genetic Validation of a Therapeutic Target in a Mouse Model of ALS Science Translational Medicine. Aug, 2014 | Pubmed ID: 25100738 DNA Methylation Dynamics of the Human Preimplantation Embryo Nature. Jul, 2014 | Pubmed ID: 25079558 FUS is Sequestered in Nuclear Aggregates in ALS Patient Fibroblasts Molecular Biology of the Cell. Sep, 2014 | Pubmed ID: 25009283 Notch Inhibition Allows Oncogene-independent Generation of IPS Cells Nature Chemical Biology. Aug, 2014 | Pubmed ID: 24952596 Ketamine Exposure in Early Development Impairs Specification of the Primary Germ Cell Layers Neurotoxicology and Teratology. May-Jun, 2014 | Pubmed ID: 24746641 Pathways Disrupted in Human ALS Motor Neurons Identified Through Genetic Correction of Mutant SOD1 Cell Stem Cell. Jun, 2014 | Pubmed ID: 24704492 Intrinsic Membrane Hyperexcitability of Amyotrophic Lateral Sclerosis Patient-derived Motor Neurons Cell Reports. Apr, 2014 | Pubmed ID: 24703839 Gene Profiling of Human Induced Pluripotent Stem Cell-derived Astrocyte Progenitors Following Spinal Cord Engraftment Stem Cells Translational Medicine. May, 2014 | Pubmed ID: 24604284 Nanog-independent Reprogramming to IPSCs with Canonical Factors Stem Cell Reports. Feb, 2014 | Pubmed ID: 24527385 Axonal Transport of TDP-43 MRNA Granules is Impaired by ALS-causing Mutations Neuron. Feb, 2014 | Pubmed ID: 24507191 How to Make Spinal Motor Neurons Development (Cambridge, England). Feb, 2014 | Pubmed ID: 24449832 Picking the Lock on Pluripotency The New England Journal of Medicine. Nov, 2013 | Pubmed ID: 24283230 The Mouse C9ORF72 Ortholog is Enriched in Neurons Known to Degenerate in ALS and FTD Nature Neuroscience. Dec, 2013 | Pubmed ID: 24185425 Derivation of Induced Pluripotent Stem Cells from the Baboon: a Nonhuman Primate Model for Preclinical Testing of Stem Cell Therapies Cellular Reprogramming. Dec, 2013 | Pubmed ID: 24182315 Opportunities and Challenges of Pluripotent Stem Cell Neurodegenerative Disease Models Nature Neuroscience. Jul, 2013 | Pubmed ID: 23799470 Modeling Human Disease with Pluripotent Stem Cells: from Genome Association to Function Cell Stem Cell. Jun, 2013 | Pubmed ID: 23746975 Directed Differentiation and Functional Maturation of Cortical Interneurons from Human Embryonic Stem Cells Cell Stem Cell. May, 2013 | Pubmed ID: 23642365 CLP1 Links TRNA Metabolism to Progressive Motor-neuron Loss Nature. Mar, 2013 | Pubmed ID: 23474986 Analysis of Human Embryos from Zygote to Blastocyst Reveals Distinct Gene Expression Patterns Relative to the Mouse Developmental Biology. Mar, 2013 | Pubmed ID: 23261930 Developmental Bias in Cleavage-stage Mouse Blastomeres Current Biology : CB. Jan, 2013 | Pubmed ID: 23177476 SnapShot: Directed Differentiation of Pluripotent Stem Cells Cell. May, 2012 | Pubmed ID: 22632979 Erosion of Dosage Compensation Impacts Human IPSC Disease Modeling Cell Stem Cell. May, 2012 | Pubmed ID: 22560080 補償がない場合の卵子提供者募集の非現実的 Cell Stem Cell. Oct, 2011 | Pubmed ID: 21982228 人間の受精卵は、マウスへの核移植後数時間以内に再プログラミングではなく Nature Communications. 2011 | Pubmed ID: 21971503 機能性脊髄運動ニューロンにマウスおよびヒトの線維芽細胞への変換 Cell Stem Cell. Sep, 2011 | Pubmed ID: 21852222 神経学的疾患の幹細胞モデルを構築し、脱構築 Neuron. May, 2011 | Pubmed ID: 21609821 体細胞は、ヒト人工多能性幹細胞に突然変異をコーディング Nature. Mar, 2011 | Pubmed ID: 21368825 ヒトESやiPS細胞の変化のリファレンス·マップは、多能性細胞株の高スループット特性を有効にする Cell. Feb, 2011 | Pubmed ID: 21295703 ヒト誘導多能性幹細胞の機能的に特徴づけ·テスト·セット Nature Biotechnology. Mar, 2011 | Pubmed ID: 21293464 幹細胞の分化に向けてエピジェネティックランドスケープダウンShushing Development (Cambridge, England). Aug, 2010 | Pubmed ID: 20554719 受容細胞核因子は核移植によってリプログラミングに必要な、 Development (Cambridge, England). Jun, 2010 | Pubmed ID: 20463036 SOX17は、直接胚の遺伝子発現を調節することにより、マウス胚性幹細胞の分化を促進し、自己更新間接的に拮抗 Genes & Development. Feb, 2010 | Pubmed ID: 20123909 患者固有の多能性幹細胞の臨床応用に向けた進展 The Journal of Clinical Investigation. Jan, 2010 | Pubmed ID: 20051636 TGF-βシグナル伝達の小分子阻害剤は、Nanogを誘導することにより、リプログラミングにSox2のを置き換え Cell Stem Cell. Nov, 2009 | Pubmed ID: 19818703 マウスの割球に染色体の転送後、再プログラミング Current Biology : CB. Aug, 2009 | Pubmed ID: 19682906 デジタルRNAアレロタイプ化は、ヒトにおいて組織特異的および対立遺伝子特異的遺伝子発現を明らかに Nature Methods. Aug, 2009 | Pubmed ID: 19620972 対象とバイサルファイトシークエンシングは、核初期化に関連付けられたDNAメチル化の変化を明らかに Nature Biotechnology. Apr, 2009 | Pubmed ID: 19330000 内部細胞塊の分離の最適なタイミングはヒト胚性幹細胞の導出の効率が向上し、兄弟の細胞株の生成を許可します。 Cell Stem Cell. Feb, 2009 | Pubmed ID: 19200798 ヒト胚性幹細胞由来運動ニューロンはALSの原因となる変異を有するグリア細胞の毒性効果に敏感である Cell Stem Cell. Dec, 2008 | Pubmed ID: 19041780 幹細胞を使用して、病気を理解するためにリプログラミング Regenerative Medicine. Nov, 2008 | Pubmed ID: 18947303 ALS患者から生成された誘導多能性幹細胞は運動ニューロンに分化させることができる Science (New York, N.Y.). Aug, 2008 | Pubmed ID: 18669821 Mediators of Reprogramming: Transcription Factors and Transitions Through Mitosis Nature Reviews. Molecular Cell Biology. Jul, 2008 | Pubmed ID: 18568039 短絡胚盤葉上層の開発 Cell Stem Cell. Aug, 2007 | Pubmed ID: 18371343 発達分裂マウスの受精卵に染色体の転送後にリプログラミング Nature. Jun, 2007 | Pubmed ID: 17554301 胚性幹細胞ベースのALSモデルにおける運動ニューロンでグリア細胞の非細胞自律的な効果 Nature Neuroscience. May, 2007 | Pubmed ID: 17435754 ドリーの遺産:人間の核移植と子どもたちのより良い医薬品 Cloning and Stem Cells. 2007 | Pubmed ID: 17386009 ヒトの治療用細胞融合の可能性 Stem Cell Reviews. 2006 | Pubmed ID: 17848721 核初期化のための転写ロジック Cell. Aug, 2006 | Pubmed ID: 16923385 大人のマウスの排卵卵子は、非循環生殖細胞に由来する Nature. Jun, 2006 | Pubmed ID: 16799565 ヒト胚性幹細胞との融合後の体細胞の核初期化 Science (New York, N.Y.). Aug, 2005 | Pubmed ID: 16123299 核クローニング、エピジェネティックリプログラミングと細胞分化 Novartis Foundation Symposium. 2005 | Pubmed ID: 16050253 嗅細胞からクローンマウス Nature. Mar, 2004 | Pubmed ID: 14990966 胚性幹細胞から胚生殖細胞と雄性配偶子の導出 Nature. Jan, 2004 | Pubmed ID: 14668819 核移植を通じて理解X染色体不活性化:用量補正をMicromanipulating Seminars in Cell & Developmental Biology. Dec, 2003 | Pubmed ID: 15015742 四倍体胚相補性によって生存雄と雌マウスにF1胚性幹細胞の分化 Methods in Enzymology. 2003 | Pubmed ID: 14696335 体細胞核からクローンマウス胚におけるOct4の関連遺伝子の不完全な再活性化 Development (Cambridge, England). Apr, 2003 | Pubmed ID: 12620990 核クローニング、幹細胞、ゲノム·リプログラミング Cloning and Stem Cells. 2002 | Pubmed ID: 12630413 胚性幹細胞と卵丘細胞核由来のクローンマウスの異常な遺伝子発現 Proceedings of the National Academy of Sciences of the United States of America. Oct, 2002 | Pubmed ID: 12235366 四倍体胚相補することで、同じ胚性幹細胞クローンに由来する雄と雌マウス Nature Biotechnology. May, 2002 | Pubmed ID: 11981557 DNMT1過剰発現は、ゲノムメチル化、インプリンティングの消失、および胚致死を引き起こす Molecular and Cellular Biology. Apr, 2002 | Pubmed ID: 11884600 ROSA26遺伝子座におけるFLPリコンビナーゼ規制LacZ発現 Genesis (New York, N.Y. : 2000). Feb, 2002 | Pubmed ID: 11857816 脳オルガノイドを派生開発人間の脳の培養幹細胞を使用してジカ ウイルス感染症をモデル化 Max R Salick*1, Michael F Wells*2,3, Kevin Eggan2,3, Ajamete Kaykas1 1Department of Neuroscience, Novartis Institutes for BioMedical Research, 2Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, 3Department of Stem Cell and Regenerative Biology and Harvard Stem Cell Institute, Harvard University JoVE 56404 Neurosciences マウス卵母細胞への核移転 Dieter Egli1, Kevin Eggan1 1Department of Molecular and Cell Biology, Harvard JoVE 116 Biologie 冷凍細胞から出発培養:ヒトES細胞 Erin Trish1, John Dimos1, Kevin Eggan1 1Department of Molecular and Cell Biology, Harvard JoVE 86 Biologie トリプシンで継代色合いヒト胚性幹細胞、線 Erin Trish1, John Dimos1, Kevin Eggan1 1Department of Molecular and Cell Biology, Harvard JoVE 49 Biologie 凍結ヒトES細胞 Erin Trish1, John Dimos1, Kevin Eggan1 1Department of Molecular and Cell Biology, Harvard JoVE 50 Biologie
脳オルガノイドを派生開発人間の脳の培養幹細胞を使用してジカ ウイルス感染症をモデル化 Max R Salick*1, Michael F Wells*2,3, Kevin Eggan2,3, Ajamete Kaykas1 1Department of Neuroscience, Novartis Institutes for BioMedical Research, 2Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, 3Department of Stem Cell and Regenerative Biology and Harvard Stem Cell Institute, Harvard University JoVE 56404 Neurosciences
マウス卵母細胞への核移転 Dieter Egli1, Kevin Eggan1 1Department of Molecular and Cell Biology, Harvard JoVE 116 Biologie
冷凍細胞から出発培養:ヒトES細胞 Erin Trish1, John Dimos1, Kevin Eggan1 1Department of Molecular and Cell Biology, Harvard JoVE 86 Biologie
トリプシンで継代色合いヒト胚性幹細胞、線 Erin Trish1, John Dimos1, Kevin Eggan1 1Department of Molecular and Cell Biology, Harvard JoVE 49 Biologie
凍結ヒトES細胞 Erin Trish1, John Dimos1, Kevin Eggan1 1Department of Molecular and Cell Biology, Harvard JoVE 50 Biologie