Michael C. Jewett Department of Chemical and Biological Engineering Northwestern University Biography Publications Institution JoVE Articles Michael C. Jewett has not added a biography. If you are Michael C. Jewett and would like to personalize this page please email our Author Liaison for assistance. Publications Deconstructing Cell-Free Extract Preparation for in Vitro Activation of Transcriptional Genetic Circuitry ACS Synthetic Biology. Feb, 2019 | Pubmed ID: 30596483 Exposed to Sublethal Levels of Hydrogen Peroxide Mounts a Complex Transcriptional Response MSystems. Sep-Oct, 2018 | Pubmed ID: 30320218 Cell-Free Synthetic Biology for Pathway Prototyping Methods in Enzymology. 2018 | Pubmed ID: 30173768 Author Correction: Single-pot Glycoprotein Biosynthesis Using a Cell-free Transcription-translation System Enriched with Glycosylation Machinery Nature Communications. 08, 2018 | Pubmed ID: 30127449 Within-Gene Shine-Dalgarno Sequences Are Not Selected for Function Molecular Biology and Evolution. Oct, 2018 | Pubmed ID: 30085185 BioBits™ Bright: A Fluorescent Synthetic Biology Education Kit Science Advances. Aug, 2018 | Pubmed ID: 30083609 BioBits™ Explorer: A Modular Synthetic Biology Education Kit Science Advances. Aug, 2018 | Pubmed ID: 30083608 Single-pot Glycoprotein Biosynthesis Using a Cell-free Transcription-translation System Enriched with Glycosylation Machinery Nature Communications. 07, 2018 | Pubmed ID: 30002445 Design of Glycosylation Sites by Rapid Synthesis and Analysis of Glycosyltransferases Nature Chemical Biology. Jun, 2018 | Pubmed ID: 29736039 Engineered Ribosomes for Basic Science and Synthetic Biology Annual Review of Chemical and Biomolecular Engineering. Jun, 2018 | Pubmed ID: 29589973 Cell-free Protein Synthesis from Genomically Recoded Bacteria Enables Multisite Incorporation of Noncanonical Amino Acids Nature Communications. 03, 2018 | Pubmed ID: 29572528 A Pressure Test to Make 10 Molecules in 90 Days: External Evaluation of Methods to Engineer Biology Journal of the American Chemical Society. 03, 2018 | Pubmed ID: 29480720 How Many Human Proteoforms Are There? Nature Chemical Biology. Feb, 2018 | Pubmed ID: 29443976 Incorporation of Nonproteinogenic Amino Acids in Class I and II Lantibiotics ACS Chemical Biology. 04, 2018 | Pubmed ID: 29439566 A Novel Framework for Evaluating the Performance of Codon Usage Bias Metrics Journal of the Royal Society, Interface. Jan, 2018 | Pubmed ID: 29386398 Toward an Orthogonal Central Dogma Nature Chemical Biology. Jan, 2018 | Pubmed ID: 29337969 A Cell-free Platform for Rapid Synthesis and Testing of Active Oligosaccharyltransferases Biotechnology and Bioengineering. 03, 2018 | Pubmed ID: 29178580 Controlling Cell-free Metabolism Through Physiochemical Perturbations Metabolic Engineering. 01, 2018 | Pubmed ID: 29155060 Diversity of Translation Initiation Mechanisms Across Bacterial Species is Driven by Environmental Conditions and Growth Demands Molecular Biology and Evolution. Dec, 2017 | Pubmed ID: 29220489 Editorial Overview: Synthetic Biology: Frontiers in Synthetic Biology Current Opinion in Chemical Biology. 10, 2017 | Pubmed ID: 29156192 A Pipeline for Studying and Engineering Single-Subunit Oligosaccharyltransferases Methods in Enzymology. 2017 | Pubmed ID: 28935112 Repurposing Ribosomes for Synthetic Biology Current Opinion in Chemical Biology. Oct, 2017 | Pubmed ID: 28869851 Development of a CHO-Based Cell-Free Platform for Synthesis of Active Monoclonal Antibodies ACS Synthetic Biology. 07, 2017 | Pubmed ID: 28350472 Cogenerating Synthetic Parts Toward a Self-Replicating System ACS Synthetic Biology. 07, 2017 | Pubmed ID: 28330337 Establishing a High Yielding Streptomyces-based Cell-free Protein Synthesis System Biotechnology and Bioengineering. 06, 2017 | Pubmed ID: 28112394 Leveraging Genome-wide Datasets to Quantify the Functional Role of the Anti-Shine-Dalgarno Sequence in Regulating Translation Efficiency Open Biology. 01, 2017 | Pubmed ID: 28100663 Translation System Engineering in Escherichia Coli Enhances Non-canonical Amino Acid Incorporation into Proteins Biotechnology and Bioengineering. 05, 2017 | Pubmed ID: 27987323 In Vitro Reconstruction of Nonribosomal Peptide Biosynthesis Directly from DNA Using Cell-Free Protein Synthesis ACS Synthetic Biology. 01, 2017 | Pubmed ID: 27478992 Yeast Knockout Library Allows for Efficient Testing of Genomic Mutations for Cell-free Protein Synthesis Synthetic and Systems Biotechnology. Mar, 2016 | Pubmed ID: 29062921 NullSeq: A Tool for Generating Random Coding Sequences with Desired Amino Acid and GC Contents PLoS Computational Biology. Nov, 2016 | Pubmed ID: 27835644 Cell-Free Synthetic Biology: Engineering Beyond the Cell Cold Spring Harbor Perspectives in Biology. Dec, 2016 | Pubmed ID: 27742731 Depletion of Shine-Dalgarno Sequences Within Bacterial Coding Regions Is Expression Dependent G3 (Bethesda, Md.). Nov, 2016 | Pubmed ID: 27605518 Cell-Free Mixing of Escherichia Coli Crude Extracts to Prototype and Rationally Engineer High-Titer Mevalonate Synthesis ACS Synthetic Biology. 12, 2016 | Pubmed ID: 27476989 Cell-free Compartmentalized Protein Synthesis Inside Double Emulsion Templated Liposomes with in Vitro Synthesized and Assembled Ribosomes Chemical Communications (Cambridge, England). Apr, 2016 | Pubmed ID: 27019994 Synthetic Biology: Tailor-made Genetic Codes Nature Chemistry. Apr, 2016 | Pubmed ID: 27001721 A Cell-free Framework for Rapid Biosynthetic Pathway Prototyping and Enzyme Discovery Metabolic Engineering. 07, 2016 | Pubmed ID: 26996382 Editorial: Transforming Biotechnology with Synthetic Biology Biotechnology Journal. Feb, 2016 | Pubmed ID: 26844671 Evolution of Translation Initiation Sequences Using in Vitro Yeast Ribosome Display Biotechnology and Bioengineering. 08, 2016 | Pubmed ID: 26757179 A Cell-free Expression and Purification Process for Rapid Production of Protein Biologics Biotechnology Journal. Feb, 2016 | Pubmed ID: 26427345 Cell-free Protein Synthesis Enables High Yielding Synthesis of an Active Multicopper Oxidase Biotechnology Journal. Feb, 2016 | Pubmed ID: 26356243 Evolution of Translation Machinery in Recoded Bacteria Enables Multi-site Incorporation of Nonstandard Amino Acids Nature Biotechnology. Dec, 2015 | Pubmed ID: 26571098 Lysate of Engineered Escherichia Coli Supports High-level Conversion of Glucose to 2,3-butanediol Metabolic Engineering. Nov, 2015 | Pubmed ID: 26428449 Robust Production of Recombinant Phosphoproteins Using Cell-free Protein Synthesis Nature Communications. Sep, 2015 | Pubmed ID: 26350765 Protein Synthesis by Ribosomes with Tethered Subunits Nature. Aug, 2015 | Pubmed ID: 26222032 Repurposing the Translation Apparatus for Synthetic Biology Current Opinion in Chemical Biology. Oct, 2015 | Pubmed ID: 26186264 Self-Assembly of Temperature-Responsive Protein-Polymer Bioconjugates Bioconjugate Chemistry. Sep, 2015 | Pubmed ID: 26083370 Energizing Eukaryotic Cell-free Protein Synthesis with Glucose Metabolism FEBS Letters. Jul, 2015 | Pubmed ID: 26054976 Implications of Macromolecular Crowding and Reducing Conditions for in Vitro Ribosome Construction Nucleic Acids Research. May, 2015 | Pubmed ID: 25897121 PH Responsive Granulocyte Colony-stimulating Factor Variants with Implications for Treating Alzheimer's Disease and Other Central Nervous System Disorders Protein Engineering, Design & Selection : PEDS. Oct, 2015 | Pubmed ID: 25877663 Improving Cell-free Protein Synthesis Through Genome Engineering of Escherichia Coli Lacking Release Factor 1 Chembiochem : a European Journal of Chemical Biology. Mar, 2015 | Pubmed ID: 25737329 High-throughput Preparation Methods of Crude Extract for Robust Cell-free Protein Synthesis Scientific Reports. Mar, 2015 | Pubmed ID: 25727242 Controlled Assembly of Artificial Protein-protein Complexes Via DNA Duplex Formation Bioconjugate Chemistry. Mar, 2015 | Pubmed ID: 25603321 Cell-free Metabolic Engineering: Biomanufacturing Beyond the Cell Biotechnology Journal. Jan, 2015 | Pubmed ID: 25319678 Characterizing and Alleviating Substrate Limitations for Improved in Vitro Ribosome Construction ACS Synthetic Biology. Apr, 2015 | Pubmed ID: 25079899 Characterizing IGR IRES-mediated Translation Initiation for Use in Yeast Cell-free Protein Synthesis New Biotechnology. Sep, 2014 | Pubmed ID: 25017988 Non-standard Amino Acid Incorporation into Proteins Using Escherichia Coli Cell-free Protein Synthesis Frontiers in Chemistry. 2014 | Pubmed ID: 24959531 Cell-free Synthetic Biology Special Issue ACS Synthetic Biology. Jun, 2014 | Pubmed ID: 24950238 The Impact of Transcriptional Tuning on in Vitro Integrated RRNA Transcription and Ribosome Construction Nucleic Acids Research. Jun, 2014 | Pubmed ID: 24792158 Quantifying Position-dependent Codon Usage Bias Molecular Biology and Evolution. Jul, 2014 | Pubmed ID: 24710515 A Combined Cell-free Transcription-translation System from Saccharomyces Cerevisiae for Rapid and Robust Protein Synthe Biotechnology Journal. May, 2014 | Pubmed ID: 24677809 Cell-free Protein Synthesis from a Release Factor 1 Deficient Escherichia Coli Activates Efficient and Multiple Site-specific Nonstandard Amino Acid Incorporation ACS Synthetic Biology. Jun, 2014 | Pubmed ID: 24328168 Substrate Replenishment and Byproduct Removal Improve Yeast Cell-free Protein Synthesis Biotechnology Journal. May, 2014 | Pubmed ID: 24323955 Mapping Condition Dependent Regulation of Lipid Metabolism in Saccharomyces Cerevisiae G3 (Bethesda, Md.). Sep, 2013 | Pubmed ID: 24062529 Optimized Extract Preparation Methods and Reaction Conditions for Improved Yeast Cell-free Protein Synthesis Biotechnology and Bioengineering. Oct, 2013 | Pubmed ID: 23832321 In Vitro Integration of Ribosomal RNA Synthesis, Ribosome Assembly, and Translation Molecular Systems Biology. Jun, 2013 | Pubmed ID: 23799452 Multiplexed in Vivo His-tagging of Enzyme Pathways for in Vitro Single-pot Multienzyme Catalysis ACS Synthetic Biology. Feb, 2012 | Pubmed ID: 22737598 Synthetic in Vitro Circuits Current Opinion in Chemical Biology. Aug, 2012 | Pubmed ID: 22676890 Cell-free Biology: Exploiting the Interface Between Synthetic Biology and Synthetic Chemistry Current Opinion in Biotechnology. Oct, 2012 | Pubmed ID: 22483202 Cell-free Synthetic Biology: Thinking Outside the Cell Metabolic Engineering. May, 2012 | Pubmed ID: 21946161 Cell-free Protein Synthesis: Applications Come of Age Biotechnology Advances. Oct, 2011 | Pubmed ID: 22008973 Precise Manipulation of Chromosomes in Vivo Enables Genome-wide Codon Replacement Science (New York, N.Y.). Jul, 2011 | Pubmed ID: 21764749 Biology by Design: from Top to Bottom and Back Journal of Biomedicine & Biotechnology. 2010 | Pubmed ID: 21052559 Update on Designing and Building Minimal Cells Current Opinion in Biotechnology. Oct, 2010 | Pubmed ID: 20638265 Escherichia coli-Based Cell-Free Protein Synthesis: Protocols for a robust, flexible, and accessible platform technology Max Z. Levine*1,2, Nicole E. Gregorio*2,3, Michael C. Jewett4,5,6,7, Katharine R. Watts2,3, Javin P. Oza2,3 1Department of Biological Sciences, California Polytechnic State University, San Luis Obispo, 2Center for Applications in Biotechnology, California Polytechnic State University, San Luis Obispo, 3Department of Chemistry and Biochemistry, California Polytechnic State University, 4Department of Chemical and Biological Engineering, Northwestern University, 5Chemistry of Life Processes Institute, Northwestern University, 6Center for Synthetic Biology, Northwestern University, 7Interdisciplinary Biological Sciences Program, Northwestern University JoVE 58882 Chimie
Escherichia coli-Based Cell-Free Protein Synthesis: Protocols for a robust, flexible, and accessible platform technology Max Z. Levine*1,2, Nicole E. Gregorio*2,3, Michael C. Jewett4,5,6,7, Katharine R. Watts2,3, Javin P. Oza2,3 1Department of Biological Sciences, California Polytechnic State University, San Luis Obispo, 2Center for Applications in Biotechnology, California Polytechnic State University, San Luis Obispo, 3Department of Chemistry and Biochemistry, California Polytechnic State University, 4Department of Chemical and Biological Engineering, Northwestern University, 5Chemistry of Life Processes Institute, Northwestern University, 6Center for Synthetic Biology, Northwestern University, 7Interdisciplinary Biological Sciences Program, Northwestern University JoVE 58882 Chimie