Tomoaki Shirao Department of Pharmacology, Graduate School of Medicine Gunma University Biography Publications Institution JoVE Articles Tomoaki Shirao has not added a biography. If you are Tomoaki Shirao and would like to personalize this page please email our Author Liaison for assistance. Publications Phldb2 is Essential for Regulating Hippocampal Dendritic Spine Morphology Through Drebrin in an Adult-type Isoform-specific Manner Neuroscience Research. Dec, 2022 | Pubmed ID: 36162735 Impairment of Ciliary Dynamics in an APP Knock-in Mouse Model of Alzheimer's Disease Biochemical and Biophysical Research Communications. Jun, 2022 | Pubmed ID: 35453040 Postsynaptic Structure Formation of Human IPS Cell-derived Neurons Takes Longer Than Presynaptic Formation During Neural Differentiation in Vitro Molecular Brain. Oct, 2021 | Pubmed ID: 34629097 Ciliary GPCR-based Transcriptome As a Key Regulator of Cilia Length Control FASEB BioAdvances. Sep, 2021 | Pubmed ID: 34485842 NMDA Receptor-dependent and -independent Effects of Natural Compounds and Crude Drugs on Synaptic States As Revealed by Drebrin Imaging Analysis The European Journal of Neuroscience. Jun, 2021 | Pubmed ID: 33851450 Genetic Knockout of the Serotonin Reuptake Transporter Results in the Reduction of Dendritic Spines in In Vitro Rat Cortical Neuronal Culture Journal of Molecular Neuroscience : MN. Nov, 2021 | Pubmed ID: 33403594 Properties of Primary Cilia in Melanin-concentrating Hormone Receptor 1-bearing Hippocampal Neurons in Vivo and in Vitro Neurochemistry International. Jan, 2021 | Pubmed ID: 33197527 Soy Isoflavones Accelerate Glial Cell Migration GPER-Mediated Signal Transduction Pathway Frontiers in Endocrinology. 2020 | Pubmed ID: 33250856 PKN1 Promotes Synapse Maturation by Inhibiting MGluR-dependent Silencing Through Neuronal Glutamate Transporter Activation Communications Biology. Nov, 2020 | Pubmed ID: 33244074 Drebrin Expression Patterns in Patients with Refractory Temporal Lobe Epilepsy and Hippocampal Sclerosis Epilepsia. Aug, 2020 | Pubmed ID: 32662890 X-irradiation of Developing Hippocampal Neurons Causes Changes in Neuron Population Phenotypes, Dendritic Morphology and Synaptic Protein Expression in Surviving Neurons at Maturity Neuroscience Research. Nov, 2020 | Pubmed ID: 31711782 High-content Imaging Analysis for Detecting the Loss of Drebrin Clusters Along Dendrites in Cultured Hippocampal Neurons Journal of Pharmacological and Toxicological Methods. 2019 | Pubmed ID: 31271780 Assessment of NMDA Receptor Inhibition of Phencyclidine Analogues Using a High-throughput Drebrin Immunocytochemical Assay Journal of Pharmacological and Toxicological Methods. 2019 | Pubmed ID: 31082488 Characterization of Functional Primary Cilia in Human Induced Pluripotent Stem Cell-Derived Neurons Neurochemical Research. Jul, 2019 | Pubmed ID: 31037609 Association Between Decreased Serum TBIL Concentration and Immediate Memory Impairment in Schizophrenia Patients Scientific Reports. Feb, 2019 | Pubmed ID: 30733572 Drebrin Isoforms Critically Regulate NMDAR- and MGluR-Dependent LTD Induction Frontiers in Cellular Neuroscience. 2018 | Pubmed ID: 30349460 CaMKIIβ is Localized in Dendritic Spines As Both Drebrin-dependent and Drebrin-independent Pools Journal of Neurochemistry. 07, 2018 | Pubmed ID: 29675826 Isoform-dependent Regulation of Drebrin Dynamics in Dendritic Spines Neuroscience. May, 2018 | Pubmed ID: 29522857 Homer, Spikar, and Other Drebrin-Binding Proteins in the Brain Advances in Experimental Medicine and Biology. 2017 | Pubmed ID: 28865024 Role of Drebrin in Synaptic Plasticity Advances in Experimental Medicine and Biology. 2017 | Pubmed ID: 28865021 Localization of Drebrin: Light Microscopy Study Advances in Experimental Medicine and Biology. 2017 | Pubmed ID: 28865017 General Introduction to Drebrin Advances in Experimental Medicine and Biology. 2017 | Pubmed ID: 28865011 Drebrin E Regulates Neuroblast Proliferation and Chain Migration in the Adult Brain The European Journal of Neuroscience. Sep, 2017 | Pubmed ID: 28833685 Drebrin Restricts Rotavirus Entry by Inhibiting Dynamin-mediated Endocytosis Proceedings of the National Academy of Sciences of the United States of America. May, 2017 | Pubmed ID: 28416666 The Role of Drebrin in Neurons Journal of Neurochemistry. Jun, 2017 | Pubmed ID: 28199019 The Role of Drebrin in Dendritic Spines Molecular and Cellular Neurosciences. Oct, 2017 | Pubmed ID: 28161364 X Irradiation Induces Acute Cognitive Decline Via Transient Synaptic Dysfunction Radiation Research. 04, 2016 | Pubmed ID: 27023259 Early-stage Development of Human Induced Pluripotent Stem Cell-derived Neurons Journal of Neuroscience Research. Dec, 2015 | Pubmed ID: 26346430 Nuclear Translocation of Calcium/Calmodulin-dependent Protein Kinase IIδ3 Promoted by Protein Phosphatase-1 Enhances Brain-derived Neurotrophic Factor Expression in Dopaminergic Neurons The Journal of Biological Chemistry. Aug, 2015 | Pubmed ID: 26163515 A Novel Role for Drebrin in Regulating Progranulin Bioactivity in Bladder Cancer Oncotarget. May, 2015 | Pubmed ID: 25839164 An Inhibitory Pathway Controlling the Gating Mechanism of the Mouse Lateral Amygdala Revealed by Voltage-sensitive Dye Imaging Neuroscience Letters. Mar, 2015 | Pubmed ID: 25646995 Comparison of the Radiosensitivities of Neurons and Glial Cells Derived from the Same Rat Brain Experimental and Therapeutic Medicine. Sep, 2014 | Pubmed ID: 25120594 Histone Deacetylase Mediates the Decrease in Drebrin Cluster Density Induced by Amyloid Beta Oligomers Neurochemistry International. Oct, 2014 | Pubmed ID: 25058791 Phosphorylation of Drebrin by Cyclin-dependent Kinase 5 and Its Role in Neuronal Migration PloS One. 2014 | Pubmed ID: 24637538 Cellular Localization and Dendritic Function of Rat Isoforms of the SRF Coactivator MKL1 in Cortical Neurons Neuroreport. May, 2014 | Pubmed ID: 24589521 Myosin II ATPase Activity Mediates the Long-term Potentiation-induced Exodus of Stable F-actin Bound by Drebrin A from Dendritic Spines PloS One. 2014 | Pubmed ID: 24465547 Spikar, a Novel Drebrin-binding Protein, Regulates the Formation and Stabilization of Dendritic Spines Journal of Neurochemistry. Feb, 2014 | Pubmed ID: 24117785 Identification, Expression and Characterization of Rat Isoforms of the Serum Response Factor (SRF) Coactivator MKL1 FEBS Open Bio. 2013 | Pubmed ID: 24251100 AMP-activated Protein Kinase Counteracts Brain-derived Neurotrophic Factor-induced Mammalian Target of Rapamycin Complex 1 Signaling in Neurons Journal of Neurochemistry. Oct, 2013 | Pubmed ID: 23841933 Actin Filaments and Microtubules in Dendritic Spines Journal of Neurochemistry. Jul, 2013 | Pubmed ID: 23692384 Selective Reduction of Drebrin and Actin in Dendritic Spines of Hippocampal Neurons by Activation of 5-HT(2A) Receptors Neuroscience Letters. Jun, 2013 | Pubmed ID: 23684573 X Irradiation Changes Dendritic Spine Morphology and Density Through Reduction of Cytoskeletal Proteins in Mature Neurons Radiation Research. Jun, 2013 | Pubmed ID: 23578130 Regulation of Myotube Formation by the Actin-binding Factor Drebrin Skeletal Muscle. Dec, 2011 | Pubmed ID: 22152295 Evidence for Cell Density Affecting C2C12 Myogenesis: Possible Regulation of Myogenesis by Cell-cell Communication Muscle & Nerve. Dec, 2011 | Pubmed ID: 22102468 Inhibitory Role of Inducible CAMP Early Repressor (ICER) in Methamphetamine-induced Locomotor Sensitization PloS One. 2011 | Pubmed ID: 21738744 Role of Cerebellar Cortical Protein Synthesis in Transfer of Memory Trace of Cerebellum-dependent Motor Learning The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Jun, 2011 | Pubmed ID: 21677179 Effectiveness of Carbon-ion Beams for Apoptosis Induction in Rat Primary Immature Hippocampal Neurons Journal of Radiation Research. 2010 | Pubmed ID: 20940520 Chemico-genetic Identification of Drebrin As a Regulator of Calcium Responses The International Journal of Biochemistry & Cell Biology. Feb, 2010 | Pubmed ID: 19948240 Effect of Radiation on the Development of Immature Hippocampal Neurons in Vitro Radiation Research. Dec, 2009 | Pubmed ID: 19929418 Drebrin a Knockout Eliminates the Rapid Form of Homeostatic Synaptic Plasticity at Excitatory Synapses of Intact Adult Cerebral Cortex The Journal of Comparative Neurology. Nov, 2009 | Pubmed ID: 19711416 Three-dimensional Distribution of Fos-positive Neurons in the Supramammillary Nucleus of the Rat Exposed to Novel Environment Neuroscience Research. Aug, 2009 | Pubmed ID: 19409426 Activity of the AMPA Receptor Regulates Drebrin Stabilization in Dendritic Spine Morphogenesis Journal of Cell Science. Apr, 2009 | Pubmed ID: 19339553 Drebrin E is Involved in the Regulation of Axonal Growth Through Actin-myosin Interactions Journal of Neurochemistry. Apr, 2009 | Pubmed ID: 19222710 Chemical and Morphological Alterations of Spines Within the Hippocampus and Entorhinal Cortex Precede the Onset of Alzheimer's Disease Pathology in Double Knock-in Mice The Journal of Comparative Neurology. Dec, 2007 | Pubmed ID: 17912741 Role of Actin Cytoskeleton in Dendritic Spine Morphogenesis Neurochemistry International. 2007 | Pubmed ID: 17590478 Drebrin a Content Correlates with Spine Head Size in the Adult Mouse Cerebral Cortex The Journal of Comparative Neurology. Aug, 2007 | Pubmed ID: 17559090 Increase in AMPA Receptor-mediated Miniature EPSC Amplitude After Chronic NMDA Receptor Blockade in Cultured Hippocampal Neurons Neuroscience Letters. May, 2007 | Pubmed ID: 17395372 Synaptic Dysfunction and Disruption of Postsynaptic Drebrin-actin Complex: a Study of Neurological Disorders Accompanied by Cognitive Deficits Neuroscience Research. May, 2007 | Pubmed ID: 17379343 Down-regulation of Drebrin A Expression Suppresses Synaptic Targeting of NMDA Receptors in Developing Hippocampal Neurones Journal of Neurochemistry. Apr, 2006 | Pubmed ID: 16635259 AMPA Receptor Downscaling at the Onset of Alzheimer's Disease Pathology in Double Knockin Mice Proceedings of the National Academy of Sciences of the United States of America. Feb, 2006 | Pubmed ID: 16492745 Differential Effects of X-irradiation on Immature and Mature Hippocampal Neurons in Vitro Neuroscience Letters. May, 2006 | Pubmed ID: 16483715 Activation of N-methyl-D-aspartate Receptor Induces a Shift of Drebrin Distribution: Disappearance from Dendritic Spines and Appearance in Dendritic Shafts Molecular and Cellular Neurosciences. Mar, 2006 | Pubmed ID: 16368245 Overexpression of Drebrin A in Immature Neurons Induces the Accumulation of F-actin and PSD-95 into Dendritic Filopodia, and the Formation of Large Abnormal Protrusions Molecular and Cellular Neurosciences. Dec, 2005 | Pubmed ID: 16456930 Overexpression of Drebrin A in Immature Neurons Induces the Accumulation of F-actin and PSD-95 into Dendritic Filopodia, and the Formation of Large Abnormal Protrusions Molecular and Cellular Neurosciences. Sep, 2005 | Pubmed ID: 16054392 [Synaptic Development and Abnormality at Protein Level] Nihon Shinkei Seishin Yakurigaku Zasshi = Japanese Journal of Psychopharmacology. Aug, 2004 | Pubmed ID: 15484828 Drebrin-dependent Actin Clustering in Dendritic Filopodia Governs Synaptic Targeting of Postsynaptic Density-95 and Dendritic Spine Morphogenesis The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Jul, 2003 | Pubmed ID: 12878700 A Novel, Brain-specific Mouse Drebrin: CDNA Cloning, Chromosomal Mapping, Genomic Structure, Expression, and Functional Characterization Genomics. May, 2002 | Pubmed ID: 11991718 Change in the Shape of Dendritic Spines Caused by Overexpression of Drebrin in Cultured Cortical Neurons The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. May, 1999 | Pubmed ID: 10234022 Modulatory Role of Drebrin on the Cytoskeleton Within Dendritic Spines in the Rat Cerebral Cortex The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Nov, 1996 | Pubmed ID: 8929425 Easy and Reproducible Low-Density Primary Culture using Frozen Stock of Embryonic Hippocampal Neurons Noriko Koganezawa1, Reiko T. Roppongi2, Yuko Sekino3,4, Izuo Tsutsui3, Ayaka Higa5, Tomoaki Shirao1,5 1Department of Pharmacology, Graduate School of Medicine, Gunma University, 2Gunma University Initiative for Advanced Research, Gunma University, 3Department of Veterinary Pathophysiology and Animal Health, Graduate school of Agricultural and Life Sciences, The University of Tokyo, 4Institute for Drug Discovery Innovation, 5AlzMed, Inc. JoVE 64872 Neurosciences
Easy and Reproducible Low-Density Primary Culture using Frozen Stock of Embryonic Hippocampal Neurons Noriko Koganezawa1, Reiko T. Roppongi2, Yuko Sekino3,4, Izuo Tsutsui3, Ayaka Higa5, Tomoaki Shirao1,5 1Department of Pharmacology, Graduate School of Medicine, Gunma University, 2Gunma University Initiative for Advanced Research, Gunma University, 3Department of Veterinary Pathophysiology and Animal Health, Graduate school of Agricultural and Life Sciences, The University of Tokyo, 4Institute for Drug Discovery Innovation, 5AlzMed, Inc. JoVE 64872 Neurosciences