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3D Co-Culture Assay: Vascularized Osteogenic Niches for Cancer Drug Screening

Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(Ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate (Video) | JoVE

Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(Ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate (Video) | JoVE

01:15 min

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Establishing the 3D Stromal Monoculture (Video) | JoVE

Establishing the 3D Stromal Monoculture (Video) | JoVE

01:46 min

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Establishing the 3D Stromal-Endothelial Cell Co-Culture (Video) | JoVE

Establishing the 3D Stromal-Endothelial Cell Co-Culture (Video) | JoVE

01:48 min

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Quantifying the Endothelial Cell Network Formation After the 3D Stromal-Endothelial Cell Co-Culture (Video) | JoVE

Quantifying the Endothelial Cell Network Formation After the 3D Stromal-Endothelial Cell Co-Culture (Video) | JoVE

02:34 min

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Assessing the Osteogenic Differentiation of 3D Stromal-Endothelial Cells by Monitoring the Alkaline Phosphatase Activity (Video) | JoVE

Assessing the Osteogenic Differentiation of 3D Stromal-Endothelial Cells by Monitoring the Alkaline Phosphatase Activity (Video) | JoVE

03:44 min

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Lisa A. Krattiger¹, Maria Mitsi², Benjamin R. Simona², Martin Ehrbar¹

¹Department of Obstetrics, University Hospital Zurich, University of Zurich, ²Ectica Technologies AG

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골수 혈관 틈새의 시험관 내 모델은 중간엽 및 내피 세포를 프리캐스트 3D PEG 하이드로겔에 파종하여 설정됩니다. 틈새의 내피 네트워크, ECM 성분 및 ALP 활성은 사용된 성장 인자에 따라 다릅니다. 이 플랫폼은 진행된 암 모델에 사용할 수 있습니다.

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