Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System

June 23rd, 2023

Author Spotlight: Establishing <em>CENP-E</em> Knockout HeLa Cells – A Novel Approach to Study Kinesin-7 <em>CENP-E</em> Biology and its Inhibitors

Author Spotlight: Establishing CENP-E Knockout HeLa Cells – A Novel Approach to Study Kinesin-7 CENP-E Biology and its Inhibitors

02:19 min

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Trasfezione, isolamento e screening delle cellule HeLa knockout <i>per CENP-E </i>

Trasfezione, isolamento e screening delle cellule HeLa knockout per CENP-E

04:10 min

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Colorazione in immunofluorescenza e microscopia confocale ad alta risoluzione di cellule HeLa knockout <i>CENP-E </i> mediate da CRISPR/Cas9

Colorazione in immunofluorescenza e microscopia confocale ad alta risoluzione di cellule HeLa knockout CENP-E mediate da CRISPR/Cas9

03:07 min

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Preparazione cromosomica e analisi del cariotipo di cellule HeLa knockout <i>CENP-E </i> mediate da CRISPR/Cas9

Preparazione cromosomica e analisi del cariotipo di cellule HeLa knockout CENP-E mediate da CRISPR/Cas9

02:19 min

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Meng-Fei Xu^1,2, Jie Chen^1,2, Yue Xu^1,2, Jing-Lian Zhang^1,2, Yi Zhou^1,2, Jie-Jie He^1,2, Shan Wu^1,2, Ya-Lan Wei^3,4, Zhen-Yu She^1,2

¹Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, ²Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, ³College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, ⁴Medical Research Center, Fujian Maternity and Child Health Hospital

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Questo articolo riporta la costruzione di cellule knockout della proteina E associata al centromero (CENP-E) utilizzando il sistema CRISPR/Cas9 e tre strategie di screening basate sul fenotipo. Abbiamo utilizzato la linea cellulare knockout CENP-E per stabilire un nuovo approccio per convalidare la specificità e la tossicità degli inibitori CENP-E, utile per lo sviluppo di farmaci e la ricerca biologica.

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Centromere-Associated Protein-E Gene Editing Using the CRISPR/Cas9 System

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