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Purification of AAV Vectors by Single-Step Heparin Affinity Chromatography

April 5th, 2024
*1,2,3, *1,2,3, 1,2,4, 1,2,5, 1,2,3, 1,2,3, 1,2,3,6, *1,2,4,5, *1,2,3,4
1CNC-UC - Center for Neuroscience and Cell Biology, University of Coimbra, 2CIBB - Center for Innovative Biomedicine and Biotechnology, University of Coimbra, 3IIIUC - Institute for Interdisciplinary Research, University of Coimbra, 4ViraVector - Viral Vectors for Gene Transfer Core Facility, University of Coimbra, 5FFUC - Faculty of Pharmacy, University of Coimbra, 6MICC-CNC - Microscopy Imaging Center of Coimbra - CNC, University of Coimbra
* These authors contributed equally

Summary
Automatic Translation

Dette manuskript beskriver en detaljeret protokol til generering og oprensning af adeno-associerede virale vektorer ved hjælp af en optimeret heparinbaseret affinitetskromatografimetode. Det præsenterer en enkel, skalerbar og omkostningseffektiv tilgang, hvilket eliminerer behovet for ultracentrifugering. De resulterende vektorer udviser høj renhed og biologisk aktivitet, hvilket beviser deres værdi i prækliniske undersøgelser.

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