Adjuvant Activity of Mycobacterium paratuberculosis in Enhancing the Immunogenicity of Autoantigens During Experimental Autoimmune Encephalomyelitis (Video) | JoVE

Davide Cossu; Yuji Tomizawa; Eiichi Momotani; Kazumasa Yokoyama; Nobutaka Hattori

doi:10.3791/65422

Immunogenicity Enhancement by Mycobacterium paratuberculosis in Autoimmune Encephalomyelitis

May 12th, 2023

Adjuvant Activity of Mycobacterium paratuberculosis in Enhancing the Immunogenicity of Autoantigens During Experimental Autoimmune Encephalomyelitis (Video) | JoVE

02:51 min

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Mycobacterial Antigen Preparation (Video) | JoVE

01:38 min

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Preparation and Injection of Myelin Oligodendrocyte Glycoprotein (MOG35-55) Emulsion (Video) | JoVE

03:05 min

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Davide Cossu^1,2, Yuji Tomizawa¹, Eiichi Momotani¹, Kazumasa Yokoyama¹, Nobutaka Hattori^1,3

¹Department of Neurology, Juntendo University, ²Biomedical Research Core Facilities, Juntendo University, ³Neurodegenerative Disorders Collaborative laboratory, RIKEN Center for Brain Science

Summary
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Here, we present an alternative protocol to actively induce experimental autoimmune encephalomyelitis in C57BL/6 mice, using the immunogenic epitope myelin oligodendrocyte glycoprotein (MOG)_35-55 suspended in incomplete Freund's adjuvant containing the heat-killed Mycobacterium avium subspecies paratuberculosis.

Article

Immunogenicity Enhancement by Mycobacterium paratuberculosis in Autoimmune Encephalomyelitis

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