Materials
Name | Company | Catalog Number | Comments |
1,2-Dioleoyl-sn-Glycero-3-Phosph–thanolamine "PE" | Avanti | 850725C | |
L-α-Phosphatidylcholine (Egg, Chicken) "PC" | Avanti | 840051C | |
L-α-Phosphatidylinositol (Liver, Bovine) "PI" | Avanti | 840042C | |
L-α-Phosphatidylserine (Brain, Porcine) "PS" | Avanti | 8400320 | |
Cardiolipin (Heart, Bovine - Sodium Salt) "CL" | Avanti | 840012C | |
Mini-extruder set | Avanti | 610023 | |
PC membrane 0.2 mM | Avanti | 610006 | |
Costar black 96 well plate | Fisher Scientific | 07-200-590 | |
Caspase-8 cleaved human BID | R&D Systems | 882-B8-050 | |
Human BCL-xL (minus C-terminus) | R&D Systems | 894-BX-050 | |
BID/PUMA BH3 domain peptides | Anaspec | 61711/62404 | |
Synergy H1 hybrid multi-mode microplate reader | BioTek | None |
References
- Chipuk, J. E., Moldoveanu, T., Llambi, F., Parsons, M. J., Green, D. R.
The BCL-2 family reunion. Mol. Cell. 37, 299-310 (2010). - Green, D. R. Apoptotic pathways: ten minutes to dead. Cell. 121, 671-674 (2005).
- Gavathiotis, E. BAX activation is initiated at a novel interaction site. Nature. 455, 1076-1081 (2008).
- Dewson, G. To trigger apoptosis, Bak exposes its BH3 domain and homodimerizes via BH3:groove interactions. Mol. Cell. 30, 369-380 (2008).
- Dewson, G. Bak activation for apoptosis involves oligomerization of dimers via their alpha6 helices. Mol. Cell. 36, 696-703 (2009).
- Gavathiotis, E., Reyna, D. E., Davis, M. L., Bird, G. H., Walensky, L. D. BH3-triggered structural reorganization drives the activation of proapoptotic BAX. Mol. Cell. 40, 481-492 (2010).
- Kuwana, T. BH3 domains of BH3-only proteins differentially regulate Bax-mediated mitochondrial membrane permeabilization both directly and indirectly. Mol. Cell. 17, 525-535 (2005).
- Chipuk, J. E. Mechanism of apoptosis induction by inhibition of the anti-apoptotic BCL-2 proteins. Proc. Natl. Acad. Sci. U.S.A. 105, 20327-20332 (2008).
- Letai, A. Distinct BH3 domains either sensitize or activate mitochondrial apoptosis, serving as prototype cancer therapeutics. Cancer Cell. 2, 183-192 (2002).
- Kuwana, T. lipids cooperate to form supramolecular openings in the outer mitochondrial membrane. Cell. 111, 331-342 (2002).
- Lovell, J. F. Membrane binding by tBid initiates an ordered series of events culminating in membrane permeabilization by Bax. Cell. 135, 1074-1084 (2008).
- Hsu, Y. T., Youle, R. J. Nonionic detergents induce dimerization among members of the Bcl-2 family. J Biol Chem. 272, 13829-13834 (1997).
- Suzuki, M., Youle, R. J., Tjandra, N. Structure of Bax: coregulation of dimer formation and intracellular localization. Cell. 103, 645-654 (2000).
- Du, H. BH3 domains other than Bim and Bid can directly activate Bax/Bak. J Biol Chem. 286, 491-501 (2011).