Materials
| Name | Company | Catalog Number | Comments |
| 253G1 human iPS cell line | RIKEN BioResource Research Center | HPS0002 | |
| 4% paraformaldehyde | FUJIFILM Wako Pure Chemical Corporation | 163-20145 | |
| Activin A Solution, Human, Recombinant | NACALAI TESQUE, INC. | 18585 | |
| Anti-Nuclei Antibody, clone 235-1 | Chemicon | MAB1281 | Antibody against human nuclear antigen |
| B-27 Supplement (50x), serum free | Thermo Fisher Scientific | 17504044 | |
| BD FACSAria III | BD Biosciences | Cell sorter | |
| CHIR-99021 | MedChemExpress | HY-10182 | |
| Ciclosporin A | FUJIFILM Wako Pure Chemical Corporation | 035-18961 | |
| Collagenase | FUJIFILM Wako Pure Chemical Corporation | 034-22363 | |
| Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix | Corning | 354230 | Gel-like basement membrane matrix |
| CultureSure Y-27632 | FUJIFILM Wako Pure Chemical Corporation | 036-24023 | ROCK inhibitor |
| Dexamethasone | FUJIFILM Wako Pure Chemical Corporation | 047-18863 | |
| Dimethyl sulfoxide (DMSO) | FUJIFILM Wako Pure Chemical Corporation | 047-29353 | |
| Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 | Thermo Fisher Scientific | A-11055 | |
| Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 546 | Thermo Fisher Scientific | A10036 | |
| Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 | Thermo Fisher Scientific | A-21206 | |
| Falcon 5 mL Round Bottom Polystyrene Test Tube, with Cell Strainer Snap Cap | Corning | 352235 | |
| Fetal Bovine Serum | Biowest | 51820-500 | |
| GlutaMAX Supplement | Thermo Fisher Scientific | 35050061 | Dipeptide L-Alanyl-L-Glutamine |
| Human/Mouse/Rat/Canine ALCAM/CD166 Antibody | R&D Systems | AF1172 | |
| Hydrocortisone 21-hemisuccinate sodium salt | Sigma-Aldrich | H2270 | |
| iMatrix-511 silk | Nippi | 892021 | |
| ImmunoBlock | KAC | CTKN001 | Blocking solution |
| ITS-G Supplement(×100) | FUJIFILM Wako Pure Chemical Corporation | 090-06741 | |
| Leibovitz's L-15 Medium | FUJIFILM Wako Pure Chemical Corporation | 128-06075 | |
| N-Hexanoic-Try-Ile-(6)-amino Hexanoic amide (Dihexa) | Toronto Research Chemicals | H293745 | |
| Polyclonal Rabbit Anti-Human Albumin | Dako | A0001 | |
| Polyoxyethylene Sorbitan Monolaurate (Tween 20) | NACALAI TESQUE, INC. | 28353-85 | |
| RPMI-1640 | FUJIFILM Wako Pure Chemical Corporation | 189-02025 | |
| Sodium L-Ascorbate | NACALAI TESQUE, INC. | 03422-32 | |
| StemFit AK02N | REPROCELL | RCAK02N | |
| TBS (10x) | NACALAI TESQUE, INC. | 12748-31 | |
| Tetramethylrhodamine, methyl ester (TMRM) | Thermo Fisher Scientific | T668 | |
| Triton X-100 | NACALAI TESQUE, INC. | 28229-25 | |
| Trypan Blue Solution | NACALAI TESQUE, INC. | 20577-34 | |
| TRYPSIN 250 | Difco | 215240 | |
| Tryptose phosphate broth solution | Sigma-Aldrich | T8159 |
References
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