Journal
/
Biologie
/
Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue

Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue

DOI:
10.3791/1444-v

•

18:17 min

•

August 06, 2009

•

, ,
1Department of Structural and Molecular Neuroscience,McLean Hospital, 2Department of Psychiatry,Harvard Medical School, 3Department of Psychiatry,Beth Israel Deaconess Medical Center

Chapitres

  • 00:05Titre
  • 01:06Introduction
  • 01:43Sectioning of Tissue and Staining of Pyramidal Neurons
  • 05:38Single Cell Laser-Capture Microdissection (LCM)
  • 10:48Isolating RNA
  • 13:39Representative Results
  • 17:38Conclusion

Summary

Traduction automatique

Traduction automatique

We describe a process using laser-capture microdissection to isolate and extract RNA from a homogeneous cell population, pyramidal neurons, in layer III of the superior temporal gyrus in postmortem human brains. We subsequently linearly amplify (T7-based) mRNA, and hybridize the sample to the Affymetrix human X3P microarray.

Tags

High Quality RNASingle Cell PopulationsGray Matter ReductionSuperior Temporal GyrusSchizophrenia PatientsGene Expression ProfilesPyramidal NeuronsCerebral CortexLaser-capture Microdissection (LCM)Histogene Staining SolutionArcturus XT LCM SystemT7-based Linear AmplificationExperion LabChip (Bio-Rad) GelElectropherogramMRNATranscript LengthMicroarraysHuman X3P GeneChip Probe ArrayAffymetrix

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/1444?article_type=v

Obtaining Eggs from Xenopus laevis Females
check_url/fr/1444?article_type=v

Neuronal Nuclei Isolation from Human Postmortem Brain Tissue
check_url/fr/1444?article_type=v

Generation of Single-Cell Suspensions from Mouse Neural Tissue
check_url/fr/1444?article_type=v

Single-cell Suction Recordings from Mouse Cone Photoreceptors
check_url/fr/1444?article_type=v

Robust Generation of Hepatocyte-like Cells from Human Embryonic Stem Cell Populations
check_url/fr/1444?article_type=v

Studying Proteolysis of Cyclin B at the Single Cell Level in Whole Cell Populations
check_url/fr/1444?article_type=v

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)
check_url/fr/1444?article_type=v

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
check_url/fr/1444?article_type=v

A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones
check_url/fr/1444?article_type=v

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Read Article