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Immunology and Infection

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JoVE Journal Immunology and Infection
Multicolor Flow Cytometry Analyses of Cellular Immune Response in Rhesus Macaques
 
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Multicolor Flow Cytometry Analyses of Cellular Immune Response in Rhesus Macaques

Article DOI: 10.3791/1743-v • 07:11 min • April 22nd, 2010
April 22nd, 2010 •
1, 1, 2, 1
1Department of Immunology, MD Anderson Cancer Center - University of Texas, 2Department of Medicine, University of Miami

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We demonstrate the utility of multicolor flow cytometry for detailed phenotypic and functional characterization of total as well as memory subsets of CD4+ and CD8+ T cells in rhesus macaques, the ideal model for HIV/AIDS vaccine studies.

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Multicolor Flow Cytometry Cellular Immune Response Rhesus Macaques HIV-AIDS Model Pathogenesis Vaccines Therapeutics Phenotypic Analysis Functional Analysis Intracellular Cytokine Production CD4+ T Cells CD8+ T Cells Memory Subsets Interferon Gamma (INF-) Interleukin (IL)-2 PMA Plus Ionomycin (PMA+I) Cytokine Profiles Memory Cells Subsets Antigen-specific CD4+ T Cells Antigen-specific CD8+ T Cells PBMC Samples HIV Envelope Peptide Cocktail Vaccine
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