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Stable Isotope Tracing & LC-MS Analysis to Measure DHODH and SDH Activities
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13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement
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Biochimie
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Journal JoVE Biochimie
13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement

13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement

Stable Isotope Tracing & LC-MS Analysis to Measure DHODH and SDH Activities

DOI:
10.3791/200017-v

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04:55 min

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May 19, 2023

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, , ,
1Program in Molecular Medicine,University of Massachusetts Chan Medical School, 2Cancer Center,University of Massachusetts Chan Medical School

Tags

13C4-aspartate13C5-glutamineStable Isotope TracingLC-MS AnalysisSDH Activity Measurement143B Human Osteosarcoma CellsCell CultureMetabolite ExtractionVacuum ConcentrationLiquid ChromatographyMass Spectrometry

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13C4-Aspartate and 13C5-Glutamine Stable Isotope Tracing, LC-MS Analysis, and SDH Activity Measurement

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