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Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nuclei RNA Sequencing (Video) | JoVE
 

Isolation of Single Nuclei from Frozen Mammalian Tissues for Single Nuclei RNA Sequencing (Video) | JoVE

Isolation and Profiling of Single Nuclei from Frozen Mammalian Tissues

Article DOI: 10.3791/200321-v • 02:02 min • July 28th, 2023
July 28th, 2023 •
1, 1, 1, 1, 1
1Roche Pharma Research and Early Development, Pharmaceutical Sciences, Roche Innovation Centre Basel

Tags

Biology Single Nucleus Sequencing Single-cell RNA Sequencing Single-nucleus RNA Sequencing Gene Expression Difficult-to-dissociate Tissues Archival Material Robotic Dissociator Tissue Homogenization Chemical Gradient Nuclei Filtering Automated Fluorescent Cell Counter Mouse Brain Rat Kidney Cynomolgus Liver Spleen Tissue
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