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JoVE Journal Neuroscience
Preparation of Mouse Brain Tissue for Immunoelectron Microscopy
 
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Preparation of Mouse Brain Tissue for Immunoelectron Microscopy

Article DOI: 10.3791/2021-v • 08:47 min • July 20th, 2010
July 20th, 2010 •
1, 2, 1
1Department of Neurobiology and Anatomy, University of Rochester, 2Douglas Mental Health University Institute

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We describe a protocol for transcardiac perfusion of mice, removal and sectioning of the brain, as well as immunoperoxidase staining, resin embedding, and ultrathin sectioning of the brain sections. Upon completion of these procedures, the immunostained material is ready for examination with transmission electron microscopy.

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Mouse Brain Tissue Immunoelectron Microscopy Transmission Electron Microscopy TEM Microglial Oligodendrocytic Astrocytic Neuronal Subcellular Compartments Dendrite Dendritic Spine Axon Axon Terminal Perikaryon Intracellular Organelles Cytoskeleton Pre-embedding Immunocytochemistry Antibody Iba1 Marker Neurotransmitter Contents Ultrastructural Localization Of Proteins Acrolein Fixative Transcardiac Perfusion Brain Sectioning Immunoperoxidase-diaminobenzidine Staining Resin Embedding Ultrathin Sectioning
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