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JoVE Journal Neuroscience
Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy
 

Whole-Brain Single-Cell Imaging and Analysis of Intact Neonatal Mouse Brains Using MRI, Tissue Clearing, and Light-Sheet Microscopy

Article DOI: 10.3791/64096-v • 08:49 min • August 1st, 2022
August 1st, 2022 •
*1,2, *1,2, 1,2, 1,2, 3, 3,7, 7, 4,5,6, 4,5,6, 3, 4,5,6, 1,2
1UNC Neuroscience Center, University of North Carolina, Chapel Hill, 2Department of Genetics, University of North Carolina, Chapel Hill, 3Department of Psychiatry, University of North Carolina, Chapel Hill, 4Center for Animal Magnetic Resonance Imaging, The University of North Carolina at Chapel Hill, 5Biomedical Research Imaging Center, The University of North Carolina at Chapel Hill, 6Department of Neurology, The University of North Carolina at Chapel Hill, 7Department of Computer Science, The University of North Carolina at Greensboro
* These authors contributed equally

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This protocol describes methods for conducting magnetic resonance imaging, clearing, and immunolabeling of intact mouse brains using iDISCO+, followed by a detailed description of imaging using light-sheet microscopy, and downstream analyses using NuMorph.

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Whole-brain Single-cell Imaging Analysis Intact Neonatal Mouse Brains MRI Tissue Clearing Light-sheet Microscopy Image Analysis Tools Quantify Cells Computational Pipeline Preprocess Images Quantify Nuclei Mouse Cortex Cell-detection Accuracy Imaging Time Computational Resources Felix Kyere Ian Curtin Graduate Students Sample Size Holder Z-dimension UltraMicroscope II Microscope Sample Cradle Screw Holder Supports Cradle Zoom Body Magnification Micrometers Per Pixel Inspector Pro Software Light Sheet Numerical Aperture Value
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