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Immunology and Infection

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JoVE Journal Immunology and Infection
Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy
 

Visualizing Cell-to-cell Transfer of HIV using Fluorescent Clones of HIV and Live Confocal Microscopy

Article DOI: 10.3791/2061-v • 13:09 min • October 7th, 2010
October 7th, 2010 •
1, 2, 2, 3, 2, 1
1Division of Infectious Diseases, Department of Medicine, Immunology Institute, Mount Sinai School of Medicine, 2NSF Center for Biophotonics, University of California, Davis, 3Structural and Computational Biology Unit, European Molecular Biology Laboratory

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This visualized experiment is a guide for utilizing a fluorescent molecular clone of HIV for live confocal imaging experiments.

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Visualizing Cell-to-cell Transfer HIV Fluorescent Clones Live Confocal Microscopy Green Fluorescent Protein Complex Cellular Processes Fluorescence Video Microscopy Human Immunodeficiency Virus Core Protein Gag Protein Infectious Molecular Clone HIV Gag-iGFP Video Confocal Microscopy Transfect Human T Cell Line Fluorescently Labeled Uninfected CD4+ T Cells Target Cells Viral Production Transport Intercellular Structures Virological Synapses Gas Permeable Imaging Chambers
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