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Journal / Biology / Isolation of Valvular Endothelial Cells
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Isolation of Valvular Endothelial Cells
 
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Isolation of Valvular Endothelial Cells

Article DOI: 10.3791/2158-v • 11:05 min • December 29th, 2010
December 29th, 2010 •
1, 1
1Department of Biomedical Engineering, Cornell University

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We provide a method for isolating and culturing pure populations of heart valve endothelial cells (VEC). VEC can be isolated from either side of the cusp or leaflet and immediately following, underlying interstitial cell (VIC) isolation is straightforward.

Tags

Isolation Valvular Endothelial Cells Heart Valves Unidirectional Blood Flow Cardiovascular System Mechanical Stresses Fibrous Tissues Valvular Endothelial Cells Interstitial Cells Repair And Remodel In Vitro Experimentation Isolation And Culture Direct Isolation Side Specific Isolation Culture Verification Of Pure Populations Enzymatic Digestion Gentle Swab Scraping Technique Surface Cells Centrifuged Into A Pellet Resuspended And Plated Collagen I Matrix VEC Phenotype Contact Inhibited Growth Endothelial Specific Markers (PECAM1/CD31) Von Willebrand Factor (vWF) Alpha-smooth Muscle Actin (α-SMA) Functional Characteristics
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