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The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker
 
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The Production of C. elegans Transgenes via Recombineering with the galK Selectable Marker

Article DOI: 10.3791/2331-v • 12:04 min • January 11th, 2011
January 11th, 2011 •
*1, *2, 2, 2
1Department of Radiation Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, 2Department of Medicine, Division of Geriatric Medicine and Pittsburgh Institute for Neurodegenerative Diseases, University of Pittsburgh
* These authors contributed equally

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The ability to produce transgenes for Caenorhabditis elegans using genomic DNA carried by fosmids is particularly attractive as all of the native regulatory elements are retained. Described is a simple and robust procedure for the production of transgenes via recombineering with the galK selectable marker.

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C. Elegans Transgenes Recombineering GalK Selectable Marker GFP Fusion Proteins Gene Regulation Expression Pattern Tandem Affinity Purification (TAP) Promoter Reporter Gene CDNA 3' Untranslated Region Alternative Promoters Worm Genomic DNA Fosmid DNA Clones E. Coli Based Recombineering Procedure GFP Insertion TAP-tag Insertion Positive Selection Negative Selection
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