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JoVE Journal Bioengineering
Microfluidic Device for Recreating a Tumor Microenvironment in Vitro
 

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

Article DOI: 10.3791/2425-v • 16:18 min • November 20th, 2011
November 20th, 2011 •
*1, *1, 1, 1
1Department of Chemical Engineering, University Of Massachusetts Amherst
* These authors contributed equally

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We present the procedure for fabrication and operation of a microfluidic device that recreates heterogeneous tumor microenvironments in vitro. The variability in apoptosis within tumor tissue was quantified using fluorescent stains and the effective diffusion coefficient of the chemotherapeutic drug doxorubicin into tumor tissue was evaluated.

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Microfluidic Device Tumor Microenvironment In Vitro Drug Delivery Systemic Clearance Heterogeneous Three-dimensional Tumor Tissues Vasculature Nutrients Viable Cells Quiescent Cells Necrotic Cells Cancer Drugs Penetration Monolayers In Vitro Model PDMS Soft Lithography Multicellular Tumor Spheroids Hanging Drop Method Rectangular Chambers Continuous Medium Perfusion Linear Gradients Fluorescent Stains Apoptosis Variability Chemotherapeutic Drug Doxorubicin Diffusion Coefficient Cell Lines
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