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Quantitative Measurement of GLUT4 Translocation to the Plasma Membrane by Flow Cytometry
 

Quantitative Measurement of GLUT4 Translocation to the Plasma Membrane by Flow Cytometry

Article DOI: 10.3791/2429-v • 05:40 min • November 7th, 2010
November 7th, 2010 •
1, 1, 1, 1
1Department of Molecular Physiology and Biophysics, Baylor College of Medicine

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This protocol describes a rapid technique to quantify the translocation of GLUT4 from the cytoplasm to the plasma membrane of cells by flow cytometry.

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Quantitative Measurement GLUT4 Translocation Plasma Membrane Flow Cytometry Glucose Energy Source Blood Concentration Insulin Release Insulin-sensitive Tissues Type-2 Diabetes Obesity Insulin Resistance Glucose Homeostasis Mechanisms Treatment Strategies GLUT Family Glucose Transporters GLUT4 Transporter Skeletal Muscle Insulin Receptor Vesicles Cytoplasm Immunocytochemistry Fluorophore-conjugated Antibodies Technique Quantify Total Amounts Of GLUT4 Translocation
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