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Bioengineering

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JoVE Journal Bioengineering
Methods Development for Blood Borne Macrophage Carriage of Nanoformulated Antiretroviral Drugs
 

Methods Development for Blood Borne Macrophage Carriage of Nanoformulated Antiretroviral Drugs

Article DOI: 10.3791/2460-v • 18:46 min • December 9th, 2010
December 9th, 2010 •
*1, *1, 1, 1, 1, 1
1Department of Pharmacology and Experimental Neuroscience, University of Nebraska Medical Center
* These authors contributed equally

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Nanoparticles of indinavir, ritonavir, efavirenz and atazanavir were manufactured using wet milling, homogenization and ultrasonication. These nanoformulations, collectively termed nanoformulated antiretroviral therapy (nanoART), assessed macrophage-based drug delivery. Monocyte-derived macrophage nanoART uptake, retention and sustained release were determined. These preliminary studies suggest the potential of nanoART for clinical use.

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Methods Development Blood Borne Macrophage Nanoformulated Antiretroviral Drugs Pharmacodynamics Bioavailability Drug Toxicities Nanomedicine ART Regimens Compliance Drug Pharmacokinetics Manufacturing NanoART Surfactant Lipid Coating Wet Milling High-pressure Homogenization Polymer Suspension Ultrasonication Physical Properties Particle Size Charge Shape Biologic Properties Cell Uptake Retention Cytotoxicity Antiretroviral Efficacy Human Monocyte-derived Macrophages (MDM) Peripheral Blood Monocytes Isolation Leukopacks Purification
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