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Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development
 

Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development

Article DOI: 10.3791/2466-v • 10:41 min • January 7th, 2011
January 7th, 2011 •
1, 2, 2, 2,3, 2,4
1Department of Veterinary Science, University of Wisconsin, Madison, 2Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, MI, 3Present address: Department of Pulmonary Medicine, University of Michigan, Ann Arbor, MI, 4Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI

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A method to deliver morpholinos directly into the zebrafish otocyst at 24hpf has been developed. Using microinjection of morpholinos into the lumen of otic vesicle and electroporation to effect penetration, we were able to bypass the effect of morpholinos on the brain and obtain effects specific to the inner ear.

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Electroporation In Vivo Transfection DNA RNA Morpholinos Zebrafish Inner Ear Development Macromolecules Electrical Current Targeted Transfection Nervous System Eye Primary Effects Secondary Effects Phalloidin Staining Acetylated Tubulin Staining Neurons Neuronal Processes Hair Cells
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