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Monitoring Dynamic Changes In Mitochondrial Calcium Levels During Apoptosis Using A Genetically Encoded Calcium Sensor
 

Monitoring Dynamic Changes In Mitochondrial Calcium Levels During Apoptosis Using A Genetically Encoded Calcium Sensor

Article DOI: 10.3791/2579-v • 06:26 min • April 1st, 2011
April 1st, 2011 •
1, 1
1Department of Neuroscience and Cell Biology, University of Texas Medical Branch

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This protocol describes a method for real-time measurement of mitochondrial calcium fluxes by fluorescent imaging. The method takes advantage of a circularly permutated YFP-based dual-excitation ratiometric calcium sensor (ratiometric pericam-mt) selectively expressed in mitochondria.

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Monitoring Dynamic Changes Mitochondrial Calcium Levels Apoptosis Genetically Encoded Calcium Sensor Intracellular Calcium Concentration Stimuli Cellular Processes Proliferation Differentiation Calcium Accumulation Pro-apoptotic Factors Cytosol Living Cells In Situ High-resolution Fluorescent Imaging Dual-excitation Ratiometric Non-ratiometric Synthetic Calcium Indicator Dyes Intracellular Calcium Signaling Cytosolic Calcium Fluxes Rhod-2 Rhod-FF Synthetic Indicators Mitochondria Repetitive Increases Mitochondrial Morphology Leak Out Of Mitochondria Long-term Experiments Genetically Encoded Calcium Indicators Green Fluorescent Protein (GFP) Aequorin
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