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Neuroscience

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JoVE Journal Neuroscience
Determination of Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons
 

Determination of Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons

Article DOI: 10.3791/2704-v • 09:56 min • May 23rd, 2011
May 23rd, 2011 •
1, 1
1Department of Molecular Pharmacology and Experimental Therapeutics, Loyola University Chicago

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We demonstrate application of the fluorescence indicator, TMRM, in cortical neurons to determine the relative changes in TMRM fluorescence intensity before and after application of a specific stimulus. We also show application of the fluorescence probe H2DCF-DA to assess the relative level of reactive oxygen species in cortical neurons.

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Mitochondrial Membrane Potential Reactive Oxygen Species Live Rat Cortical Neurons Respiratory Chain ATP Generation Oxidative Stress Environmental Toxins Oxidative Phosphorylation Mitochondrial Dysfunction Fluorescent Probes Rhodamine 123 TMRM TMRE JC-1 Fluorescence Indicators Dihydroethidium Dihydrorhodamine 123 H2DCF-DA Fluorescence Intensity
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