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Immunology and Infection

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JoVE Journal Immunology and Infection
Methods to Assess Beta Cell Death Mediated by Cytotoxic T Lymphocytes
 
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Methods to Assess Beta Cell Death Mediated by Cytotoxic T Lymphocytes

Article DOI: 10.3791/2724-v • 12:13 min • June 16th, 2011
June 16th, 2011 •
1, 1, 1
1Departments of Pathology, Immunology and Laboratory Medicine, College of Medicine, University of Florida

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Cell-mediated lymphocytotoxicity (CML) assays can be used to test autoreactive responses and study mechanisms of cell death in vitro. However, using live-cell confocal microscopic imaging techniques with fluorescent dyes, the type and kinetics of cell death as well as the pathways utilized can be studied in greater detail.

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Beta Cell Death Cytotoxic T Lymphocytes Type 1 Diabetes Insulin Production Mechanisms Of Beta Cell Death Cell-mediated Lymphocytotoxicity Assay 51Cr Release Effector Cells Autoreactive CD8+ Cytotoxic T Lymphocytes AI4 T Cell Receptor Specific Lysis Mitochondria Mitochondrial Functional Changes Mitochondrial Membrane Potential Dye
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