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Immunology and Infection

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JoVE Journal Immunology and Infection
A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies
 

A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies

Article DOI: 10.3791/56256-v • 04:47 min • February 23rd, 2018
February 23rd, 2018 •
1,2, 1, 1,2, 3
1Department of Microbiology, Icahn School of Medicine at Mount Sinai, 2Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, 3Department of Infectious Disease, J. Craig Venter Institute

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We describe a method to measure the activation of Fc-mediated effector functions by antibodies that target the influenza virus hemagglutinin. This assay can also be adapted to assess the ability of monoclonal antibodies or polyclonal sera targeting other viral surface glycoproteins to induce Fc-mediated immunity.

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Fc-mediated Effector Functions Influenza Hemagglutinin Specific Antibodies Assess Activation Antibody-dependent Immunity Immunology Vaccinology Technique 24 Hours Material Preparation Data Analysis Mark Bailey Graduate Student Peter Palese Laboratory Transfection DNA Coding Viral Hemagglutinin Transfection Reagent Optimum-reduced Serum Medium Infection A549 Cells
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