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Immunology and Infection

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JoVE Journal Immunology and Infection
Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins
 

Immuno-fluorescence Assay of Leptospiral Surface-exposed Proteins

Article DOI: 10.3791/2805-v • 10:47 min • July 1st, 2011
July 1st, 2011 •
1,2, 3,4
1Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, 2Research service, 151, Veterans Affairs Greater Los Angeles Healthcare System, 3Departments of Medicine, Urology at David Geffen School of Medicine and Department of Microbiology, Immunology and Molecular Gentics, University of California Los Angeles (UCLA), 4Division of Infectious Diseases, 111F, Veterans Affairs Greater Los Angeles Health Care System

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résumé
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An efficient method to assess surface-exposure of leptospiral proteins is described. The method is specifically designed to avoid disruption of the fragile outer membrane of leptospiral cells. This technique requires employment of several negative controls to assess the integrity of the outer membrane and specificity of antibody reaction.

Tags

Immuno-fluorescence Assay Leptospiral Surface-exposed Proteins Bacterial Surface Proteins Cellular Localization Functional Role Virulence Factors Pathogenicity Mechanisms Fractionating Leptospiral Membranes Outer-membrane Proteins (OMPs) Lipoproteins Transmembrane OMPs Hydrophobic Interaction Lipid Bilayer Phospholipids Amphipathic β-sheets Barrel-like Structure Outer Membrane Integrity Immunofluorescence Assay (IFA)
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