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Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice
 

Hyperinsulinemic-euglycemic Clamps in Conscious, Unrestrained Mice

Article DOI: 10.3791/3188-v • 11:10 min • November 16th, 2011
November 16th, 2011 •
1, 2,3, 3, 2,3, 3, 4, 2,3, 2,3
1Diabetes and Obesity Research Center, Sanford-Burnham Medical Research Institute at Lake Nona, 2Department of Molecular Physiology and Biophysics, Vanderbilt University School of Medicine, 3Vanderbilt Mouse Metabolic Phenotyping Center, Vanderbilt University School of Medicine, 4Department of Pediatrics and Cellular and Integrative Physiology, Indiana University School of Medicine

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résumé
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The hyperinsulinemic-euglycemic clamp, or insulin clamp, is the gold standard for assessing insulin action in vivo. A method for performing insulin clamps in mice is described. This includes a method for arterial catheterization that permits experiments to be performed in conscious, unrestrained mice with minimal stress.

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Hyperinsulinemic-euglycemic Clamps Insulin Action Type 2 Diabetes Insulin Infusion Glucose Infusion Glucose Infusion Rate (GIR) Whole-body Insulin Action Isotopic 2[14C]deoxyglucose Tissue-specific Glucose Uptake [3-3H]glucose Endogenous Glucose Appearance (endoRa) Hepatic Glucose Production Whole-body Glucose Disappearance (Rd) Genetic Mouse Models Of Metabolic Disease Diabetes Research Insulin Clamp Performance
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