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JoVE Journal Neuroscience
Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient
 
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Preparation of Synaptoneurosomes from Mouse Cortex using a Discontinuous Percoll-Sucrose Density Gradient

Article DOI: 10.3791/3196-v • 08:31 min • September 17th, 2011
September 17th, 2011 •
1, 1, 2, 1
1Department of Pathology and Laboratory Medicine, Waisman Center for Developmental Disabilities, University of Wisconsin, 2Department of Biochemistry, Waisman Center for Developmental Disabilities, University of Wisconsin

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A method to prepare translationally active, intact synaptoneurosomes (SNs) from mouse brain cortex is described. The method uses a discontinuous Percoll-sucrose density gradient allowing for the quick preparation of active SNs.

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Synaptoneurosomes Mouse Cortex Discontinuous Percoll-Sucrose Density Gradient Homogenization Fractionation Resealed Vesicles Isolated Terminals Synaptic Transmission Pre- And Postsynaptic Characteristics Neuronal Signaling Neurotransmitters Ficoll Cytotoxic Centrifugation Filtration Mechanical Damage Percoll-sucrose Gradient Method Translationally Active SNs Isotonic Conditions
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