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JoVE Journal Neuroscience
Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient
 

Preparation of Synaptic Plasma Membrane and Postsynaptic Density Proteins Using a Discontinuous Sucrose Gradient

Article DOI: 10.3791/51896-v • 08:06 min • September 3rd, 2014
September 3rd, 2014 •
*1, *1, 1, 1
1Department of Pharmacology and Toxicology, University of Toronto
* These authors contributed equally

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résumé
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This article details the enrichment of proteins associated with the synaptic plasma membrane by ultracentrifugation on a discontinuous sucrose gradient. The subsequent preparation of post-synaptic density proteins is also described. Protein preparations are suitable for western blotting or 2D DIGE analysis.

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Synaptic Plasma Membrane Postsynaptic Density Sucrose Gradient Neuronal Subcellular Fractionation Synaptic Protein Isolation Membrane Protein Isolation Postsynaptic Density Protein Isolation Western Blotting 2D DIGE
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