Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Simultaneous Two-photon In Vivo Imaging of…
JoVE Journal
Neuroscience
Author Produced

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Neuroscience
Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex
 

Simultaneous Two-photon In Vivo Imaging of Synaptic Inputs and Postsynaptic Targets in the Mouse Retrosplenial Cortex

Article DOI: 10.3791/53528-v • 16:45 min • March 13th, 2016
March 13th, 2016 •
*1, *1, 2, 1, 3
1Department of Molecular and Cellular Neuroscience, Nencki Institute of Experimental Biology, 2Department of Biochemistry, Nencki Institute of Experimental Biology, 3Neurobiology Centre, Nencki Institute of Experimental Biology
* These authors contributed equally

Chapitres

résumé
Automatic Translation

This video shows the craniotomy procedure that allows chronic imaging of neurons in mouse retrosplenial cortex using in vivo two photon microscopy in Thy1-GFP transgenic line. This approach is combined with injection of mCherry-expressing adeno-associated virus into dorsal hippocampus. These techniques allow long-term monitoring of experience-dependent structural plasticity in RSC.

Tags

Keywords: Two-photon Imaging In Vivo Retrosplenial Cortex Craniotomy Adeno-associated Virus Structural Plasticity GFP MCherry Stereotactic Frame Isoflurane Anesthesia Inflammation Pain Infection
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter