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JoVE Journal Neuroscience
Two-photon Imaging of Cellular Dynamics in the Mouse Spinal Cord
 

Two-photon Imaging of Cellular Dynamics in the Mouse Spinal Cord

Article DOI: 10.3791/52580-v • 10:44 min • February 22nd, 2015
February 22nd, 2015 •
1, 2, 4, 3, 1, 5, 2
1Molecular Biology and Biochemistry, University of California, Irvine, 2Physiology and Biophysics, University of California, Irvine, 3Neurobiology and Behavior, University of California, Irvine, 4University of California San Francisco Diabetes Center, University of California, San Francisco, 5Pathology, University of Utah

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A new ex vivo preparation for imaging the mouse spinal cord. This protocol allows for two-photon imaging of live cellular interactions throughout the spinal cord.

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Two-photon Imaging Cellular Dynamics Mouse Spinal Cord Live-cell Imaging Neural Precursor Cells Neuroinflammatory Demyelinating Disorders Axonal Damage Oligodendrocytes Remyelination Therapeutic Treatment Chronic Demyelinating Diseases Ventral Side Of The Spinal Cord Intravital Imaging Histochemical Staining Approaches Transplanted Cells Endogenous Cells Enhanced Yellow Fluorescent Protein-expressing Axons Enhanced Green Fluorescent Protein-expressing Transplanted NPCs
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