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In Situ Hybridization for the Precise Localization of Transcripts in Plants
 

In Situ Hybridization for the Precise Localization of Transcripts in Plants

Article DOI: 10.3791/3328-v • 12:15 min • November 23rd, 2011
November 23rd, 2011 •
1, 1, 1
1Cold Spring Harbor Laboratory

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The in situ hybridization protocol described here allows a direct localization of mRNA and small RNA expression at the cellular level with high sensitivity and specificity. The procedure is optimized for paraffin-embedded plant tissue sections, is applicable to a wide range of plants and tissues, and can be completed within ten days.

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In Situ Hybridization Precise Localization Transcripts Plants Genomics Research Plant Biology Gene Expression Microarray Next Generation Sequencing Developmental Processes Physiological Processes Stress Response Processes Epigenetic Pathways Small RNA Pathways Gene Regulatory Networks Spatiotemporal Resolution Profiling Method Laser Microdissection Fluorescence-activated Cell Sorting Biological Role Of A Gene Spatiotemporal Pattern Of Expression Cellular Resolution Development Environmental Stimuli Mutants Analysis Transgenic Reporter Lines
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