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Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells
 

Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber

Article DOI: 10.3791/3415-v • 14:18 min • February 13th, 2012
February 13th, 2012 •
1, 2, 3
1Department of Physiology and Biophysics, Confocal Microscopy and Cell Imaging Core, Robert Wood Johnson Medical School, 2Department of Physiology and Biophysics, Robert Wood Johnson Medical School, 3Muscle Biology Research Group-MUBIG Schools of Nursing & Medicine, University of Missouri-Kansas City

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The extent of store-operated Ca2+ entry (SOCE) can be monitored using fluorescent Ca2+ indicators. Mn2+ quenching of such indicators assays SOCE in cultured cells and skeletal muscle fibers. A technique allowing spatial and temporal resolution of SOCE by confocal imaging of mechanically skinned muscle fibers is also described.

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Fluorescence-based Measurement Store-operated Calcium Entry Live Cells Cultured Cancer Cell Skeletal Muscle Fiber SOCE Capacitative Ca2+ Entry Endoplasmic Reticulum Sarcoplasmic Reticulum Second Messenger Cellular Processes Proliferation Apoptosis Gene Transcription Motility Epithelial Cells Patch-clamp Studies Intracellular Ca2+ Measurements High-throughput Screening Fluorescence Methods
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