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JoVE Journal Neuroscience
Methods for Study of Neuronal Morphogenesis
 

Methods for Study of Neuronal Morphogenesis: Ex vivo RNAi Electroporation in Embryonic Murine Cerebral Cortex

Article DOI: 10.3791/3621-v • 10:12 min • May 18th, 2012
May 18th, 2012 •
1,2,3, 1,2, 1,2, 1,2,3
1Department of Molecular, Cellular Biology and Biochemistry, Brown University, 2Institute for Brain Science, Brown University, 3Department of Psychiatry and Human Behavior, Warren Alpert School of Medicine, Brown University

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résumé
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To conduct a rapid assessment of the function of genes in the development of cerebral cortex, we describe methods involving the ex vivo electroporation of plasmids co-expressing inhibitory RNA (RNAi) and GFP in murine embryonic cortex. This protocol is amenable to the study of various aspects of neurodevelopment such as neurogenesis, neuronal migration and neuronal morphogenesis including dendrite and axon outgrowth.

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Neuronal Morphogenesis Ex Vivo RNAi Electroporation Embryonic Murine Cerebral Cortex Cerebral Cortex Higher Cognitive Functions Six Layered Structure Inside-first Outside-last Manner Neuronal Migration Regulation Of Neuronal Morphogenesis Primary Cultures Tissue Environments Organotypic Slices PSilencer Modified Vector U6 Promoter Double Stranded Hairpin RNA CMV Promoter GFP Protein Neurite Outgrowth Candidate Genes Mosaic Analysis
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